PERSONAL DE APOYO
ASENSIO Cristian Jorge Alejandro
congresos y reuniones científicas
Título:
INTRACELLULAR CHLORIDE ACTS AS A SECOND MESSENGER FOR CFTR MODULATING IL-1β EXPRESSION
Autor/es:
M. CLAUZURE; M. MASSIP COPIZ; CONSUELO MORI; CRISTIAN JORGE ALEJANDRO ASENSIO; VERONICA SOTOMAYOR; A. G. VALDIVIESO; T. A. SANTA COLOMA
Lugar:
BUENOS AIRES
Reunión:
Congreso; LXII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2017
Institución organizadora:
SAIC
Resumen:
The CFTR protein is a cAMP-regulated chloride channel. By usingdifferential display, we have found previously several CFTR-dependentgenes, including c-Src, MUC1, MTND4 and CISD1. WeGLRX5 and RPS27. Here, using nigericin and tributyltin to clampthe pH and the intracellular chloride concentration [Cl-]i of IB3-1 epithelialcells, we show that IL-1β is also a Cl--dependent gene thatcan be modulated by changing the intracellular chloride concentration,in a biphasic way. The IL-1β secretion measured by ELISAalso showed a similar pattern of response to changes in [Cl-]i. Themechanism involves an IL-1β autocrine effect, since in the presenceof the IL-1β receptor antagonist IL1RN and anti-IL-1β blocking antibody,Cl- effects disappeared (p
