Natural compounds present in scutellaria baicalensis and rosmarinus officinalis induce cell cycle arrest in human endometrial stromal cells and mast cell lines.

FERELLA L; BASTON JI; OLIVARES C; MC CORMACK B; SIMITSIDELLIS I; COLLINS F; SAUNDERS PTK; MERESMAN G

Ciudad Autónoma de Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Current medical therapies available for endometriosis (EDT) are often unacceptable due to their long term side effects. Natural compounds are being investigated because of their potential beneficial impacts on symptoms of cancer and EDT. Wogonin (WG) is a flavonoid isolated from the Scutellaria baicalensis root. Carnosic Acid (CA) and Rosmarinic Acid (RA) are antioxidant compounds found in Rosmarinus officinallis leaves. Our previous studies demonstrated that these compounds inhibit endometrial stromal cell proliferation. Our aim was to evaluate the effect of WG, CA and RA on cell cycle progression in human endometrial stromal cell lines (T-HESC) and a human mast cell line (HMC1.1) with the latter chosen because of the association of mast cells with pain in inflammatory conditions such as EDT. T-HESC cells were incubated with WG (40, 80µM), CA (2.5, 5µg/ml) or RA (50;100µg/ml) or the appropriate vehicle control for 48hs. Cell cycle distribution was assessed by flow cytometry. WG 40µM and CA 2.5µg/ml decreased the percentage of T-HESC cells in G0/G1 and increased the percentage of cells in G2/M phase (p