Dams' exposure time to n-6 exceeded diet differentially affects the intrauterine development of mouse

SOLÍS MR; SANTILLÁN ME; MOLINA R; STUTZ G; BIANCONI S

Jornada; XVIII Jornadas de Investigación Científica; 2017

SECyT-FCM-UNC

Environmental factors during fetal and neonatal life are crucial for the developmental outcomes. Exposure to adverse conditions tends to prepare thebody for a postnatal development according to such limitations. Excess of n-6 fatty acids during pregnancy affects the development of fetuses. Hypothesis:the longer exposure of the mother to a linoleic acid exceeded diet, the major alterations on the development of their offspring.Objective: to assess theintrauterine development of fetuses from dams fed n-6 exceeded diets during different exposition times. Adult female mice received commercial diet (C, n= 8) or C supplemented with 10% of sunflower oil from 25 days prior (G25, n = 8) or from the beginning of (G, n = 8) gestation. Assessed: body weightand daily food intake evolution. On 16.5 gestational day, pregnant mothers were sacrificed to calculate: reproductive success (RS) and ovulation rate. Wealso recorded: ovaries and uteri weights. Corpus luteum (CL) were counted and fetal-placental units (FPU), placentas and fetuses were weighed. Infetuses, it was assessed: vitality, weight, length and general characteristics (presence of: meconium -M-, fatty yolk sac -FYS- lips, nostrils, tail, ribs, limband fingers). Cumulative food intake was significantly different between the groups: G25 (57.10±2.11g) vs. G (71.20±1.75g) vs. C (80.39±1.9), p C (p