Consequences of maternal fructose intake on browning potential of retroperitoneal adipose tissue from adult offspring.

SPINEDI E; GIOVAMBATTISTA A; ALZAMENDI A

CABA

Congreso; LXII Reunion de la Sociedad Argentina de Investigacion Clinica (SAIC); 2017

SAIC

Beige adipocytes are highly able to dispel chemical energy as heat through uncoupling protein-1 (UCP1) activity. Cold exposure or β3 adrenergic agonist treatment stimulates white to beige adipocyte conversion. Our aim was to assess whether maternal FRD intake during pregnancy affects the development of the browning capacity of WRPAT progenitor cells from the adult male offspring. On pregnancy day 1, dams were provided with either tap water alone (control) or FRD (10 % w/v; in tap water) and fed ad libitum with chow up to delivery. Weaned pups received water and chow ad libitum up to 60 days of age (experimental day). C and F indicate pups born to control and FRD dams, respectively. On experimental day, WRPAT pad was dissected and stromal vascular fraction cells (SVFs) were isolated. mRNA expression levels of beige and white adipogenic markers were assessed in WRPAT pads and SVFs. These cells were cultured and several differentiation markers were quantified (qPCR). Previous own studies indicate that pre-natal nutritional intervention decreased the adipogenic potential of adult WRPAT precursor cells, favoring hypertrophic mass expansion and a distorted adipokine secretion pattern. In F WRPAT pads we now found lower (p