Previous studies from our group indicate that FRD consumption during gestation induces a decrease in adipose precursor cells (APCs) from retroperitoneal adipose tissue (RPAT). This was accompanied by a reduced local RPAT mass in the first adult male proge

ALZAMENDI A; GAMBARO S; MIRANDA JR; SPINEDI E; GIOVAMBATTISTA A

Orlando

Congreso; 2017 ENDO Meeting; 2017

ESO

Previousstudies from our group indicate that FRD consumption during gestation induces adecrease in adipose precursor cells (APCs) from retroperitoneal adipose tissue(RPAT). This was accompanied by a reduced local RPAT massin the first adult male progeny. These changes favor for adipocyte hypertrophyand distorted pattern of leptin secretion. We now evaluated the impact of FRD intakeby the gestating dams on the adipogenic capacity of stromal vascular fraction(SVF) cells from RPAT of their first adult male progeny. On pregnancy day 1,dams were provided with either tap water alone (control) or containing fructose(10% w/v in drinking water; FRD) and fed ad libitum with chow, up to delivery.Lactating dams and their pups (between 21 and 60 of age) received water andchow ad libitum. C and F animals were born tocontrol and FRD dams, respectively. At adult life (age 60 days) male rats weresacrificed and RPAT pads were dissected, then SVF cells were isolated. Trunkblood was collected to corroborate hyperleptinemia.  The mRNA expression levels of adipogenicpotential markers were assessed by qPCR in RPAT SVF cells. PPARγ expression was quantified in differentiating cells, byimmunofluorescence. Terminal differentiation was evaluated by Papanicolaustaining and expression markers by qPCR. Also, RPAT UCP-1 expression level in thewhole tissue was assessed. Our data indicated that RPAT SVF cells from F rats expressedlower and higher (p<0.05) levels of CD34 and Pref-1, respectively; althoughno changes in PPARγ, Zfp423 and Wnt-10b expression were noticed, a fact suggestingdecreased adipogenic capacity in F SVF cells. Immunofluorescence analyses forPPARγ (on day 4 post-differentiation) showed a lower number of positive cells andPPARγ intensity per cell in F than C RPAT (p<0.05), thus confirming datafrom SVF cell PPARg expression levels mentioned above. On day 10 post-differentiation, alower number of mature adipocyte was found in the F group (p<0.05 vs C)being cell adiponectin expression levels lower in F cells (p<0.05 vs C), agreeingwith data from SVF cells and in cells on day 4 post-differentiation. Increased ob, LPL and FAS gene expression wasfound in RPAT pads from F rats (p<0.05), a tissue showing dysfunctional hypertrophicadipocytes. Moreover, RPAT UCP-1 expression level was lower in F animals (p<0.05vs C), possibly indicating an alteration in RPAT thermogenic function. Theseresults indicate that in uteromaternal intervention (FRD-intake) induces permanent alterations in maleoffspring?s RPAT development and function when examined at adult life. A fact manifestedby decreased adipogenic potential of RPAT APCs, favoring hypertrophic RPAT massexpansion and inhibition of local adipogenesis. (PICT-2013-0930/CICPBA/FPREDM052015).