Antioxidant effects of metformin plus ITS during in vitro maturation of porcine COCs

LUCHETTI, CG.; LOMBARDO, DM.

Ciudad Autónoma de Buenos Aires

Otro; Reunión Conjunta de Sociedades de Biociencias; 2017

Sociedad Argentina de Biología - Sociedad Argentina de Investigación Clínica

The insulin-sensitizing drug metformin has antioxidant properties in a variety of models. Lee et al. (2005) have shown that metformin supplementation during in vitro culture of porcine embryos increases the blastocyst rate considerably. Insulin- transferrin-selenium (ITS) is commonly added to oocyte in vitro maturation (IVM) media because it increases the maturation rate and has antioxidant effects. We aimed to determine if metformin added to IVM media containing ITS has antioxidant effects. Porcine COCs were obtained by follicular aspiration of slaughterhouse ovaries and matured in vitro in the supplemented M199 medium during 46-47 h. The medium was added to ITS (I) or ITS + metformin (I+M). COCs were pooled (10 COCs per tube, seven tubes per group) and homogenized in PBS on the ice and centrifuged at 4500 x g. Supernatants were stored at -80°C until use. Lipid peroxidation was determined by TBA-RS and total glutathione was measured by a colorimetric assay, both techniques adapted to 96 Well microplates. Lipid peroxidation decrease in the group I+M compared to group I (p