INVESTIGADORES
LOMBARDO Daniel Marcelo
congresos y reuniones científicas
Título:
Neurotrophin receptors expression in Caiman latirostris thymus
Autor/es:
GAUNA AÑASCO, LG.; DI MATTEO, AM.; SOÑEZ, MC.; GAZZANEO, P.; LOMBARDO DM.
Lugar:
Buenos Aires
Reunión:
Jornada; Primer Encuentro Rioplatense De Biologia - XIV Jornadas Anuales De La Sociedad Argentina De Biología. Fundación Pablo Cassará; 2012
Institución organizadora:
Sociedad Argentina De Biología.y Fundación Pablo Cassará
Resumen:
Neurotrophins (NTs) are growth factors that act in
nervous system and also in lymphatic system of domestic animals. The objective
of this work was to determine the location of high affinity receptors for NTs
in thymus of Caiman latirostris
compared with other domestic and wild animals (Astianux fasciatus, pigeon, broiler chicken, Chaetoprhactus vellerosus, pig, cow, horse, Lama glama and human. 14 thymuses were fixed in 10% buffered
formalin and processed with histological routine technique, embedded in
parafin. On serial sections an indirect immunocytochemical method (ABC) was
applied using anti-TrkA, B and C, diluted 1:100. In the negative controls
primary antibody incubation was omitted. TrkA expressed in type VI reticular epithelial
cells as in Astianux fasciatus,
broiler chicken, Chaetophractus sp,
pig, cow, Lama glama and human. TrkB
was identified in dendritic cells of the cortico-medullary region as in pigeon.
TrkC was localized in type VI reticular epithelial cells as in Astianux fasciatus and Chaetoprhactus sp. As in other
vertebrate species, NTs receptors were identified
in the thymus cythoreticulum cells, suggesting a possible paracrine role of NGF
(TrkA), BDNF (TrkB) and NT3 (TrkC) growth factors on the lymphocyte maturation.