APOPTOTIC PROCESSES IN BURSA RELATED TO VACCINATION AGAINST INFECTIOUS BURSAL DISEASE

KILIAN, MJ.; BOVIEZ, JD.; GAMBAROTTA, MC.; LOMBARDO, DM.

Tandil

Jornada; Primeras Jornadas de Ciencias Morfológicas de las Sierras de Tandil. Universidad del Centro de la Provincia de Buenos Aires (UNCPBA).; 2011

Universidad del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias.

Infectious bursal disease is a viral disease of young birds that causes apoptosis in the B lymphoid series, leading to immunosuppression. The application of live virus vaccines have been helpful but its apoptotic effects in Fabricious bursa are unknown. This  research studied the apoptotic effects of such vaccines on this organ. We worked  on 220 one-day  broilers, divided into four experimental groups. Two groups were vaccinated with live vaccine strain intermediate soft Lukert and intermediate strain ST-14 respectively. A third group was inoculated with the adjuvant vaccines. The fourth control group received no treatment. Animals were sacrificed in groups to 3-7-14-21-28 days post inoculation. Bursas of Fabricius were analyzed by staining with hematoxylin and eosin, and TUNEL technique for the quantification of apoptotic cells and degree of bursal damage. Data  was analyzed by analysis of variance for p <0.05. Vaccinated animals were found to have increased  their apoptotic cells in each sample period compared to those of the control animals, and the pathological analysis showed obvious damage. The live vaccine virus, such as the strains used in the formulation of these vaccines, trigger mechanisms in the bursa like the pathogenic virus, leading to the death of B lymphocytes by apoptotic phenomena. Key words:Apoptosis-Bursa of Fabricius-Infectious Bursitis