Evaluación radiológica de la densidad mineral ósea (DMO) en ratas ovariectomizadas )OVX) tratadas con monofluorofosfato (MFP) y zoledronato (Z)

LOMBARTE, MERCEDES; MORENO HILDA; RIGALLI ALFREDO; DI LORETO VERÓNICA

Rosario

Congreso; XXIX Reunión Anual de la Sociedad de Biología de Rosario.; 2009

Sociedad de Biología de Rosario

&lt;!-- @page { margin-right: 2.54cm; margin-top: 2.54cm; margin-bottom: 1.27cm } P { margin-bottom: 0.21cm } --&gt; RADIOLOGICAL ASSESSMENT OF TRABECULAR BONE MINERAL DENSITY IN OVARIECTOMIZED RATS TREATED WITH MONOFLUOROPHOSPHATE (MFP) AND ZOLEDRONATE (Z) Lombarte M, Moreno H, Rigalli A, Di Loreto V. Facultad Ciencias Médicas. UNR. We have demostrated the decrease in bone mass and bone volume (BV/TV) histomorphometrically measured in ovarictomized (OVX) rats. These changes were only prevented by Z. Radiological fotodensitometry is a simple technique to estimate bone mineral. The aim of this work was to study if the trabecular bone mineral density (BMDt) measured by X-ray fotodensitometry is a suitable technique to assess bone changes in OVX rats treated with MFP, Z and MFP+Z. Twenty-four 70-day-old Sprague Dawley rats were subjected to OVX: 1) Controls, 2) MFP: 40 umol/100 g bw/d per os, 3) Z: 1.5 ug/kg/w sc, 4) MFP+Z: 40 umol/100 g bw/d per os+1.5 ug/kg/w sc. Six rats were sham operated (sham). After 30 days, rats were eutanized, tibias were obtained, defleshed and radiographed together with and aluminium stepwedge reference. BMDt was measured at proximal metaphysis level over digitals images with a propre image analysis program. Results were reported as g of bone mineral Ca/cm2. Data were compared with Kruskall-Wallis and Dunn’s test.  Z prevent the decrease k BMDt induced by OVX. (Sham: 22.7±1.3, OVX: 17.7±1.5*, MFP: 17.2±1.0*, Z: 22.8±1.1, MFP±Z: 21.6±0.5. * indicates differences compared to sham, p<0.05, mean±SE). Histomorfometrically, Z prevented the decrease in BV/TV by OVX(BV/TV: Sham: 31.8[28.9-61.0], OVX: 22.5[16.5-28.7]*, MFP: 16.2[14.3-20.2]*, Z:62.9[46.9-66.1]*, MFP±Z:50.4[43.5-54.3], p<0.05, median[range]) We found a significant correlation between BMD and BV/TV (Spearman, p<0.001). We concluded that BMDt measured by X-ray is a suitable technique to estimate quantitative bone changes and can be advantageous used in a non invasive way.