Variaciones en el perfil proteico de músculo de la quela de Cyrtograpsus angulatus y Chasmagnathus granulatus de la laguna de Mar Chiquita (Pcia. Bs As) en respuesta a la salinidad ambiental.

PINONI, S. A. Y LÓPEZ MAÑANES, A. A.

Rosario, Argentina

Congreso; VII Congreso-XXV Reunión Anual de la Sociedad de Biología de Rosario; 2005

SBR

We have previously shown differential responses of alkaline phosphataseactivities and an increase of Na+/K+ATPase activity in chelamuscle of C. angulatus and C. granulatus upon hypo-osmotic stress.The aim of this work was to study the protein profile of chelamuscle of both crabs in different salinities. Male adults were acclimatedfor 15 (t15) or 33 (t33) days to 35‰ salinity (S) and 15days at 10‰S (C.angulatus) or 38-40 days to 35‰ or 10‰S(C.granulatus). An aliquot (20μg proteins) of muscle homogenates(0.25M Sucrose/0.5mM EGTA-Tris pH 7.4) was loaded into nativepolyacrilamide gels and PAGE-SDS gels (ßmercaptoethanol).(Molecular weight (MW) standards: carbonic anhydrase for nativegels; Sigma standards mixture for PAGE-SDS). Proteins werestained with Brilliant Blue Coomasie R-250.. In C. angulatus uponosmoconforming conditions (35‰S) occurred a decrease by t33in several bands (Rf=0.045; 0.134; 0.239; MW:180-84 kD) and anincrease in bands with MW lower than 37 kD. In 10‰S a band ofRf= 0.239 and bands with MW 180-84 kD were increased by t33.In C.granulatus in 10‰S occurred an enhancement in bands withRf= 0.022-0.134 and Rf=0.239 and MW between 180-84 kD and48-37 kD. Our results show the occurrence of variations in proteinprofile in chela muscle upon hypo-osmotic stress which could berelated to adjustments (i.e. cell volume regulation, mobilizationsof energy substrates) secondary to osmo-ionoregulation. Whetherincreases in high molecular proteins are related with a synthesis denovo of Na+/K+ATPase remains to be investigated.