EFECTO DEL HIPOTIROIDISMO SOBRE GENES RELACIONADOS AL METABOLISMO DE COLESTEROL EN HÍGADO Y CORAZÓN DE RATON

LASCANO C, GATICA LV, ALVAREZ SM, GIMÉNEZ MS.

Bariloche- Río Negro (Argentina)

Congreso; XXXIX Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2003

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB)

It is known that hypothyroidism condition produces several effects on lipid metabolism. In the present work we studied biochemical parameters of lipids and the expression of SREBP-2, HMG CoA Reductase and LDL Receptor genes. Male adult Balb/c mice received 10 mg of Metimazol (Danantizol)/100 ml tap water by 30 days (HT group). Control mice received tap water without the drug. Mice were killed by decapitation and blood and tissues were collected. We measured total cholesterol (CT) in liver and heart homogenates by Zak´s method. 1 µg of RNA, extracted by TRIZOL method, was transcribed to cDNA at 42°C using random hexamers as primers and MMLV-RT (Moloney Murine Leukemia Virus). Aliquots of 2 µl of cDNA were used in the amplifications by PCR using specific primers. Beta actin was used as an internal control. In HT mice, CT of liver and heart increased in relation to control. In liver, gene expression of SREBP-2 did not show any changes while HMG CoA Reductase and LDL Receptor decreased. The same situation was observed in heart. With the dosis of Metimazol used, cholesterol metabolism is affected in both organs.