PARÁMETROS DE ESTRÉS OXIDATIVO ASOCIADOS A LA DEFICIENCIA DE VITAMINA A EN AORTA DE RATA

GATICA LV, GÓMEZ NN, OLIVEROS LB, GIMÉNEZ MS

Bariloche- Río Negro (Argentina)

Congreso; XXXIX Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2003

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB)

We had demonstrated increased expression of iNOS, eNOS, COX- 2 and NF-kB in rat aorta under vitamin A deficiency. In this work we study the effect of such deficiency on antioxidant defense system in rat aorta. Wistar male 21 days old rats were fed during three months with free vitamin A diet (-A) and the same diet plus 8 mg of Retinol palmitate/kg of diet (+A). A group of -A rats received control diet fifteen days before sacrifice (-A refeeded). Serum TBARS and aorta SOD, GPx and CAT activities were measured. –A group showed increased serum TBARS and decreased SOD, GPx and CAT activities in aorta when compared to +A group. The restitution of Vitamin A to the Vit A deficient diet reverted TBARS levels and GPx activity to control values while SOD activity was partially reversed. No change on CAT activity was found. Vitamin A deficiency affects oxidative stress parameters in serum and rat aorta. Nutritional Vitamin A administration to –A rats reverts significantly those effects.