EXPRESIÓN DE PPARs y TGF-beta 1 EN AORTA DE RATAS DEFICIENTES EN VITAMINA A

GATICA LV, ALVAREZ SM, OLIVEROS LB, GIMÉNEZ MS

Iguazú, Misiones (Argentina)

Congreso; XL Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2004

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB)

We have previously shown that Vitamin A deficiency is associated with an increase of oxidative stress and inflammation in rat aorta and alteration in the lipid content of serum and aorta tissue. PPARs and TFG-beta play an important role in vascular events during progression of atherosclerosis. The aim of the present study was to examine the effects of Vitamin A deficiency on the expression of the genes of PPARalpha and PPARgamma and TGF-beta1 protein expression in rat aorta. Wistar male 21 days old rats were fed during three months with free vitamin A diet (-A) and the same diet plus 8 mg of Retinol palmitate/kg of diet (+A). Total RNA was isolated with TRIzol. Rat aorta proteins were resolved by SDS-PAGE (10%). TGF-beta1 was identified by Western blot with a specific antibody and bands were revealed using Vectastain ABC detection kit. Analysis of mRNA expression with reverse transcription-polymerase chain reaction (RT-PCR) for PPARs and protein levels for TGF-beta1 revealed higher expressions in –A aortas when compared to +A. In the present study, we show that Vitamin A deficiency regulates positively the transcription of PPARs and TGF-beta1 expression in rat aorta. These results suggest that the balance between these mediators is involved in the mechanism that probably modulates inflammatory responses in this experimental model.