PERFIL DE FOSFOLÍPIDOS Y EXPRESIÓN DE ENZIMAS INVOLUCRADAS EN EL METABOLISMO DE LOS LÍPIDOS EN AORTA DE RATA. EFECTO DE LA DEFICIENCIA EN VITAMINA A

GATICA LV, OLIVEROS LB, GIMÉNEZ MS

Pinamar, Buenos Aires (Argentina)

Congreso; XLI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB).; 2005

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB)

Antioxidants are known to reduce cardiovascular events by preventing oxidative modification of low density lipoproteins. We have previously reported the prooxidative effect and increased triglycerides, cholesterol and phospholipid contents in aorta of vitamin A deficient-rats. Here, we evaluate the phospholipid profile and mRNA expression of regulatory enzymes of aorta lipid synthesis by RT-PCR. A group of Wistar male 21 d old rats were fed during three months with a free vitamin A diet (-A) and another one with the same diet plus 8 mg of retinol palmitate/kg of diet (+A). A group of -A rats was refed with control diet fifteen days before sacrifice. Individual phospholipids were separated by TLC using silica gel H plates and chloroform/methanol/water (65:25:4 by vol.) as solvent system before quantifying by Rousser method. Total RNA was isolated using TRIzol. Aorta of -A rats showed an increase of PC, PI+PS, and PE, a decrease of SM, and no change in PG. The mRNA of diacylglycerol acyltransferase-1, CTP: phosphocholine cytidylyltransferase-a, lipoproteinlipase and lectin-like oxidized LDL receptor- 1 increased in -A aortas in relation to +A. The restitution of vitamin A to the -A rats reverted all the changes observed. Thus, the vitamin A deficiency alters the aorta phospholipid pattern and expression of lipid regulatory enzymes.