ISCHEMIC POSTCONDITIONING: DIFFERENCES BETWEEN FEMALE AND MALE

FANTINELLI JC; CALDIZ, CI; GONZÁLEZ ARBELÁEZ LF; SCURI, S; CIOCCI PARDO, A; MOSCA SM

Rio de Janeiro

Congreso; 38th World congress of International Union of Physiological Sciences; 2017

Brazilian Society of Physiology

Our objective was to determine the effects of ischemic postconditioning (IPC) on postischemic alterations in male (M) and female (F) rat hearts. The experiments performed with isolated hearts were: 1) Non-ischemic control (NIC): perfusion for 110 min; 2) Ischemic control (IC): 30-min global ischemia (GI) and 60-min reperfusion (R); 3) IPC: 3 cycles of 30 sec of GI and 30 sec of R at the beginning of R. Infarct size (IS) was measured. Systolic function was assessed by developed pressure of left ventricle (LVDP) and dP/dtmax; diastolic function by the -dP/dtmax and left ventricular end diastolic pressure (LVEDP). The expression of P-Akt, P-GSK-3β, and P-PKCε and superoxide production (SP), were determined at 3 min. of R. In IC, IS was lesser in F than M (29 ± 2 % and 40 ± 4 %, respectively, p < 0.05). At the end of R, LVDP, +dP/dtmax and -dPdtmax were higher in F than M (approximately 20 % and 55 %, respectively, p < 0.05). LVEDP was 26 ± 8 mmHg for F and 50 ± 4 mmHg for M (p < 0.05). The expression of phosphorylated kinases in NIC hearts was higher in F than M and its level increased in both sexes after GI-R. SP was lesser in F than M in all groups. After GI-R, SP increased in both sexes. IPC decreased IS and SP in F and M, improved the post-ischemic recovery of myocardial function and produced additional increase of kinases expression only in M.