Pseudomonas stutzeri as a promising Plant Growth-Promoting Bacteria for soybean in saline soils

LAMI MJ; COSTA SB; ZENOFF AM; CARAM C; ESQUIVEL-COTE R; P.A. VINCENT; ESPINOSA URGEL M; DE CRISTÓBAL RE

Cordoba

Congreso; SAIB; 2016

Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular

Pseudomonas stutzeri as a promising Plant Growth-Promoting Bacteria for soybean in saline soilsLami MJ1, Costa SB1, Zenoff AM1, Caram C1, Esquivel-Cote R2, Vincent PA1, Espinosa Urgel M3 & de Cristóbal RE1.1INSIBIO CONICET-UNT, Arg. 2Microbiología, Edafología, Col. Postgraduados, Mex. 3EEZ, Granada EspañaBacteria were isolated from roots of a plant, Sesuvium edmonstone, that grows well in the Salinas Grandes, province of Santiago del Estero. One of the most salt tolerant isolates was identified as Pseudomonas stutzeri after sequencing its 16S rDNA and was named PS19. We characterized biochemical properties of PS19 in regards of its Plant Growth-Promoting ability, studying the auxin and siderophore production, phosphate solubilization, biological nitrogen fixation, desaminase ACC activity and its growth on saline conditions. In addition, we evaluated the PS19 beneficial effect on soybean germination in saline condition assays (100mM NaCl). We used two soybeans seeds varieties, Munasqa and S100, which have different saline susceptibilities. The germination percentage, vigor and growth parameters were evaluated. For both soybean varieties, improved values in the measured parameters were obtained for the inoculated seeds. These results indicate that PS19 is a promising bacterium to enhance growth and to help the plant deal with high soil salinity.