Immunolocalization of insulin-like growth factor binding protein-2 (IGFBP-2) in bovine cystic follicles

RODRÍGUEZ F, BALDI C, ORTEGA H, BARBEITO C, GIMENO E, REY F

Aguas de Lindaio Sao Paulo. Brasil

Congreso; XIV ENAPAVE Encuentro de Patologia Veterinaria; 2009

Asocciaciao Brasileria de Patologia Veterinaria

Immunolocalization of insulin-like growth factor binding protein-2 (IGFBP-2) in bovine cystic follicles Fernanda Rodríguez, Cecilia Baldi, Hugo Ortega, Claudio Barbeito, Eduardo Gimeno, Florencia Rey Departamento de Ciencias Morfológicas, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral. Instituto de Patología. Facultad de Ciencias Veterinarias. Universidad Nacional de La Plata. Argentina. A widespread reproductive disorder in cattle is the cystic ovarian disease (COD) and the mechanism leading to their development was proposed to be of multifactorial etiology. Although it is widely accepted that a dysfunction of the hypothalamic-pituitary-gonadal axis is an important etiological factor, several reports have discussed the association between COD and growth factors in different species. The insulin-like growth factor (IGF) system has already been shown to play a key role in the regulation of proliferation and differentiation of granulosa and theca cells. IGF binding proteins (IGFBPs) regulate the availability of IGFs to their target cells by either inhibiting or potentiating their action and also can sequester extracellular IGFs and hence reduce their bioavailability. Two mechanisms have been suggested by which availability of IGFs can be modified; through the activity of specific IGFBP proteases, or by changes in the IGFBPs expression. In previous studies we detected a significant low expression of IGFBP-2 in follicular fluid and evidence exists to suggest that could be due, in part, to changes in local synthesis. In the present study we proposed to analyze the mRNA expression of IGFBP-2 by RT-PCR using GAPDH as housekeeping. Moreover, we assessed the protein localization by immunohistochemistry. Ovaries were collected at a local abattoir and dissected healthy follicles were classified into small (<<metricconverter w:st="on" productid="ಔ䐘ヶ墄ಔ㪘Ǐ겛뛳ꇅ䌈牼곤ዚ㋸˭先犀Ƕ侐喰Ǻ冸㌕6㌗退傭싟ƾ਀䢈乇NJ6㍵㎼Ď㏃ᨀ Ǫ凈鍈䧐枨鍈僰往㋸˭先鍈瑥㬱s Ɛàಈಈಈಈ婠ಔಈಈ崠ಔಈ彀ಔ夘ಔಈಈ닰ಓಈ炰ಔಈಈ憠ಔ媠ಔ죀ಓ子ಔಈಈಈಈಈಈ帐ಔ廐ಔ怰ಔ惈ಔ慠ಔ扐ಔ拨ಔ提ಔ撀ಔ斠ಔ暐ಔ材ಔ桰ಔ检ಔ榀ಔ橀ಔ櫰ಔ殠ಔ池ಔ涐ಔ満ಔ澠ಔ焠ಔ燐ಔ皰ಔ睠ಔ砐ಔɘ౬ʨ౬̠౬Ͱ౬π౬А౬नಘীಘ઀ಘଘಘரಘైಘഈಘචಘุಘ໸ಘྸಘ茀ಔ莘ಔ萰ಔ蓈ಔ蕠ಔ蘠ಔ蚸ಔ蝐ಔ蟨ಔ袀ಔ褰ಔ觰ಔ誠ಔ譐ಔ谀ಔ貰ಔ赠ಔ踐ಔ軐ಔ辀ಔ逰ಔ郠ಔ醐ಔ鉀ಔ鋰ಔ鎠ಔ鑠ಔ锠ಔ闐ಔ隀ಔ靀ಔ韰ಔ颠ಔ饠ಔ騠ಔ髐ಔ鮐ಔ鱀ಔ鴀ಔ鶰ಔ鹰ಔ鼠ಔ鿐ಔꂀಔꅀಔꇰಔꊰಔꍠಔꐠಔꓠಔꖐಔꙀಔ꛰ಔꞠಔꡐಔ꤀ಔ꧀ಔꪀಔꭀಔ꯰ಔ겠ಔ굠ಔ긠ಔ껐ಔ꾐ಔ끀ಔ냰ಔ놠ಔ뉐ಔ대ಔ뎰ಔ둠ಔ딐ಔ뗀ಔ뙰ಔ뜠ಔ럐ಔ뢀ಔ뤰ಔ맠ಔ몠ಔ뭐ಔ밀ಔ벰ಔ뵠ಔ븐ಔ뻀ಔ뽰ಔ쀠ಔ샐ಔ솀ಔ숰ಔ신ಔ쎐ಔ쑀ಔ쓰ಔ얠ಔ왐ಔ윀ಔ잰ಔ졠ಔ줐ಔ지ಔ쩰ಔ쬠ಔ쯐ಔ첀ಔ촰ಔ췰ಔ캠ಔ콠ಔ퀐ಔ탐ಔ톀ಔ툰ಔ틠ಔ펐ಔ푀ಔ픀ಔ헀ಔ홰ಔ휰ಔ퟈ಔಔಔಔಔಔಔಔಔಔಔಔಔಔಔಔಔಔಔಔಔಔಔಔಔಔಔಔಔಔsŧ䢈嗐儨ū嗰嘐喰ů沞៫薑麢ۆꔌ᤾꽴꾔푙ꏘ#ē 嘰嚐嗐ė ᐰࠆثԁ܅Ѓࠆثԁ܅ă#ě Deutsche Telekom Root CA 1ă噐6ꏘ#嘐ć舰舰䄄ΠĂȂ㜄퉊ぃ؍⨉䢆čԁ脰㇃》؉唃؄ȓ单ᐱሰ̆ѕጊ䔋瑮畲瑳渮瑥㬱㤰̆ѕጋ眲睷攮瑮畲瑳渮瑥䌯卐椠据牯⹰戠⁹敲⹦⠠楬業獴氠慩⹢ㄩ〥أ唃଄ᰓ挨
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