Tissue specific global gene expression profile in Yerba Mate (Ilex paraguariensis).

R KRISHNA SHRESTHA; J. FAY; C WATKIN ; S. LITWINIUK; L. TALAVERA STEFANI; C. ARGÜELLES; MM. MIRETTI

Foz do Iguazú, Paraná Brasil

Congreso; 11th International Congress of Plant Molecular; 2015

Sociedad Brasilera de Genteica

Transcriptome data provide direct insight into the global gene expression at specific time and space.Yerba Mate (Ilex paraguariensis, Aquifoliaceae) represents the foremost regional economy produce innortheast Argentina. However, it was not until recently that has been granted access to biotechnologyand genetic studies applied to Yerba Mate (YM) production. Here we present the first tissue specifictranscription profile in YM. Tissue samples were stored in liquid nitrogen until RNA extraction. Total RNAextracted from leaves and roots was quality-assessed and 500ng was used for the RNA-Sequencinglibrary construction (100bp paired-ends reads) in triplicates. Libraries were then sequenced in twoIllumina HiSeq 2500 lanes. Denovo-assembly from sequence reads was done using the Trinity assembler.Short contigs were filtered out and incomplete genes were extended to full-length RNA transcripts usingthe software Full-lengther based on full-length UniprotKB plant proteins. We obtained 119,285 proteinsequences, though only 24,612 were complete. Assembled sequences were used as reference sequenceand tissue specific sequence reads were then mapped to the reference sequence using bowtie2.Sequencing three libraries per sample gave consistency to our results. Analyses from the previouslyavailable transcriptome data are based on a single RNA-Seq library, and therefore prone toinconsistencies. Our results represent the first step to tissue specific expression profiling in YM, essentialto explore effects of diseases, abiotic stress and microorganism interactions on YM production. Furtherinvestigations are necessary to expand these analyses covering other tissues and conditions.0761