INVESTIGADORES
ZELADA Alicia Mercedes
congresos y reuniones científicas
Título:
Small RNAs transcriptome of the Fern Pleopeltis minima (Polypodiaceae) Offers Insight into the Evolution of the MicroRNA Repertoire in Land Plants
Autor/es:
BERRUEZO FLORENCIA; DE SOUZA FLÁVIO S J; PICCA PABLO; NEMIROVSKY SERGIO; MARTINEZ TOSAR LEANDRO; RIVERO MERCEDES; MENTABERRY ALEJANDRO N; ZELADA A
Lugar:
Rosario
Reunión:
Simposio; Simposio de Genòmica Funcional de Plantas; 2017
Institución organizadora:
IBR-CONICET
Resumen:
In the last decade, genome and smallRNA (sRNA) sequencing of several plant species have helped unveil the evolutionary history of land plants. Among these, the fern group (monilophytes) occupies a key phylogenetic position, as it represents the closest extant cousin taxon of seed plants, i.e. gymno- and angiosperms. However, in spite of their evolutionary, economic and ecological importance, no fern genome has been sequenced yet and few genomic resources are available for this group. Here, we sequenced the small RNA fraction of an epiphytic South American fern, Pleopeltis minima (Polypodiaceae), and compared it to plant miRNA databases, allowing for the identification of miRNA families that are shared by all land plants, shared by all vascular plants (tracheophytes) or shared by euphyllophytes (ferns and seed plants) only. Using the recently described transcriptome of another fern, Lygodium japonicum, we also estimated the degree of conservation of fern miRNA targets in relation to other plant groups. Our results pinpoint the origin of several important miRNA families in the land plant evolutionary tree and are a resource for future genomic and functional studies of fern miRNAs.