Placental Trypanosoma cruzi infection is restricted by nitric oxide production by endothetial nitric oxide synthase isoform

M.F. TRIQUELL; E. BENIZIO; M.J. MOREIRA-ESPINOZA; J,C.RAMIREZ; L.MEZZANO; G.M. MAZZUDULI

Congreso; SLIMP/ SCHCF 2017 Latin American Society for Maternal Fetal Interaction and Placenta; 2017

Human placenta avoids Trypanosoma cruzi (T. cruzi) infection by different mechanisms and the production of nitric oxide (NO) is one of them.Objective: To elucidate the importance of NO production in the parasitic load in placental tissue and the relevance of endothelial nitric oxide synthase (eNOS) isoform as a major NO producer.Methods: Placentas from caesarean delivery from clinically and serologically healthy women and Trypomastigotes of T. cruzi isolated from a congenital case (named Lucky) were used. Placental villi explants were co-cultured in RMPI 1640 for 24 h with or without (control) 1 x106 trypomastigotes of Lucky (TcII -VI strains). Cultures were treated with NOS inhibitor NG-nitro-L-arginine methyl ester (L-NAME) at 0.1 and 1 mM. Quantification of eNOS expression by immunohistochemistry and Western blot, nitrite level in media supernatant with Griess technique, and parasitic load by qPCR with Taqman probes were assayed.Results: Placental explants in the presence of T. cruzi showed a significant increment of eNOS protein abundance (7.2 ± 0.94 fold, P