Participation of the RhD protein in the erythrocyte senescence process.

TRUCCO BOGGIONE, CAROLINA; GARCÍA BORRAS SILVIA; ENSINCK, MARÍA ALEJANDRA; MATTALONI SM; CARLOS COTORRUELO; MUFARREGE NICOLAS; LUJÁN BRAJOVICH M; BIONDI CLAUDIA

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Congreso; LXIV Reunión científica anual, Sociedad Argentina de Inmunología; 2016

Adult red blood cells (RBCs) undergo multiple changes while they age in vivo. Some of these involve modifications at membrane level and the loss of membrane with some proteins by vesiculation. These changes result in events that trigger removal of Senescent (Se) RBCs. In newborn RBCs, the senescence process is not well characterized. The aim of this study was to evaluate the participation of the RhD protein in the senescence process. Adult (n=22) and cord (n=20) RhD positive RBC suspensions were incubated with IgM anti-RhD and subsequently with APC labeled anti-µ. RhD negative samples were used as negative controls. SeRBC and YRBC populations were separated by flow cytometry using forward scatter and side scatter parameters. Indirect fluorescence intensity (IFI) was measured to detect anti-RhD bound to the RBC membrane surface. Paired samples t-test showed that the average of the median values of IFI associated with RhD protein expression both in adult and cord YRBCs was significantly higher when compared with SeRBCs (adult YRBC=706.0±213.79 vs SeRBC=557.5±178.88, p