Study the Biological Activities of extract from Atriplex undulata (Moq) D. Dietr. (Chenopodiaceae)

RODRIGUEZ, S.; LEIRO J.M.; MURRAY A.P; SUEIRO R. A

cancun

Conferencia; 3rd Natural Products Conference: Interface of Science and Technology as Applied to Natural Product Research; 2017

Fusion conference

Atriplex undulata, commonly known as Zampa crespa, is an endemic species. It originates from Patagonia Argentine and is used as astringent and antiequimotic.This study aims to evaluate the broad spectrum of the chemical composition and the biological properties of dichloromethane sub-extract of Atriplex undulata (Moq) D. Dietr. (Chenopodiaceae). This sub-extract was fractionated by silica gel column chromatography, for isolation and purification of the active compounds.For this, the chemical composition of the dichloromethane sub-extract of A. undulata (DCM) was determined by GC-MS and tandem mass spectrometry and identified by comparison of their 1H and 13C NMR data reported in the literature. This presented phenolic compounds, norisoprenoids, and terpenes as their major class compounds. The isolated compounds, 3-hydroxy-β-damascone and dihydro-3-oxo-β-ionol were evaluated in different biological resulting very active activities.The antimicrobial activity was accessed in gram-positive and gram-negative bacteria and in fungi, isolated from different biological fluids and reference strains. The DCM and isolated compounds were active against all microorganisms and showed antioxidant activity by scavenging free radicalsThe anti-inflammatory effect of these compounds was evaluated by measuring release of interleukins (IL-1β), tumor necrosis factor (TNF-α), expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) by reverse transcriptase-polymerase chain reaction (RT-PCR).The inhibition of mutagenicity of the sub-extract and the components isolated were evaluated using the Ames assay with 5 strains of Salmonella Typhimurium in the absence and presence of exogenous metabolic activation (rat liver fraction S9), against different mutagens.The inhibition produced by compounds isolated from the DCM from A. undulata, primarily the norisoprenoides, could be interesting for the prevention of these diseases.Taken together, these results show that dichloromethane sub- extract of Atriplex undulata has important therapeutic activities, which suggests its potential application in the pharmaceutical industry, as well as in health foods, beverages, and nutritional supplements.