PREVENTIVE EFFECT OF A PROBIOTIC ADMINISTRATION ON THE METABOLIC ALTERATIONS CAUSED BY A FRUCTOSE RICH DIET INTAKE

ZUBIRÍA, MARÍA GUILLERMINA; GAMBARO SABRINA ELIANA; CARASI, PAULA; RUMBO M.; SERRADELL, MARÍA; GIOVAMBATTISTA, ANDRÉS

Mar del plata

Encuentro; LXI Reunion Cientifica Annual de la Sociedad Argentina de Investigacion Clinica; 2016

Sociedad Argentina de Investigacion clinica

There is large evidence that gut microbiota modulates meta-bolic disorders associated Yith obesity. 9e evaluated the pre-ventive effect of a probiotic Lactobacillus administration on the metabolic alterations caused by a fructose rich diet F4&. Four different SYiss mice groups Yere studied n, CT4 mice Yere given tap Yater, F4& mice Yere given Y/v fructose in the drinMing Yater, and tYo similar groups that received by gavage 10 8 CF7 of .actoDacillus Mefiri C+&CA every hs CT4LM or F4&LM, respectively. Caloric intaMe and body Yeight Yere recorded every hs. After YeeMs, mice Yere euthanized and CT4LM and revert to control levels in F4&LM group 2., Yhile blood glucose did not display any difference betYeen groups. The m40A expression of functional adipose tissue genes 1b, L2L, Adiponectin, )24 and proinflammatory genes +0FJand TNFα in EAT Yere assessed. All these genes Yere higher in F4& mice, but Yere reverted Yith the lactobacillus administration F4&LM, 2.. For the S8F from F4& mice, Yhere the immune cells are in high proportion, the expression levels of +0FJ and TNFαYere higher 2. vs CT4 and CT4LM, Yhile for F4&LM the expression values Yere similar to CT4 mice. 9ith this preliminary results Ye can state that the administration of .. Mefiri prevents the deleterious effects of a F4&, impacting the Yeight gain and the functional profile and proinflammatory state of the EAT.plasma samples Yere collected. Epididymal adipose tissue EAT Yas dissected, Yeighted and processed for q2C4 and cellular analysis. Stromal vascular fraction S8F cells Yere isolated from EAT and processed for gene expression analysis. F4& and F4&LM shoYed an increased in caloric intaMe 2. vs. CTR and CT4LM, but only the F4& mice shoYed an increase in Yeight gain and EAT mass 2. vs. CTR, CTR-Lk and FRD-Lk). Plasmatic triglycerides levels Yere higher in F4& mice 2. vs CT4 and