Characterization of Delta to Theta Transitions in the Hippocampus and Lateral Habenula Following Mechanic or Optogenetic Stimulation in Anethetized Rats

DEMIÁN GARCÍA VIOLINI; ALEJO MOSQUEIRA; PATRICIO COLMEGNA; JOAQUIN PIRIZ; MARIANO BELLUSCIO; RICARDO SÁNCHEZ PEÑA

Mar del Plata

Congreso; XXX Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias; 2015

Sociedad Argentina de Investigación en Neurociencias

Based on experimental data, the correlation between the activation of the Diagonal Brocca Band (DBB) and the transition from delta to theta state is sought. The experiments have considered two different stimulations, exogenous and endogenous (pulse wave), namely an optogenetic activation of DBB and a nociceptive stimulus given by a tail pinch. The light stimulation is a pulse wave parameterized by its frequency, pulse width and duration. In addition, the local field potential (LFP) of the Lateral Habenula (LHb) and the hippocampus (HPC) have been recorded in order to determine the switch between states. The experimental data has been classified to obtain the following information:* Relation between the frequency content of the LHb and/or the HPC and the anesthesia effect on the specimen (delta power) * For the same input, how delta power influences the transitions from delta to theta and theta to delta, e.g. the dwell time in theta, excitation length to achieve a switch, etc* Relation between the input stimulation and the switch from delta to thetaThe method used here is based on a Fourier analysis and signal processing techniques applied to the experimental data. The final objective is to establish a correlation between the input signal characteristics (frequency, pulse width, duration) and delta power with the transitions from delta to theta in both the LHb and HPC.