CHANGES IN THE CARBOHYDRATE EXPRESSION IN CATTLE AND BUFFALOES INFECTED WITH THE ABORTIGENIC PROTOZOAN NEOSPORA CANINUM

CASPE SG, CAMPERO C, MOORE DP , CAMPERO C, ORTEGA MORA LM, BACIGALUPE D, LISCHINSKY LH, SALA JM, VENTURINI C , KONRAD JL, BARBEITO CG

Puerto Varas

Congreso; Joint Meeting 2017 Latin American Society for Maternal Fetal Interaction and Placenta (SLIMP) Sociedad Chilena de Ciencias Fisiológicas (SCHCF)Puerto Varas. Chile; 2017

Latin American Society for Maternal Fetal Interaction and Placenta (SLIMP) Sociedad Chilena de Ciencias Fisiológicas (SCHCF)

Carbohydrate pattern in the placenta was studied in different speciesshowing a great variation between species. The sacharydes of the surfaceglycoconjugates are important to the adhesion between conceptus anduterus. Besides, reproductive diseases as tritrichomonosis and campylobacteriosisgenerate changes in this pattern in cattle.Objective: The aim of this study was to characterize the saccharide inplacenta of cattle and buffaloes after experimental infection with theabortigenic protozoan Neospora caninum (N. caninum) at early gestation bylectinhistochemistry.Methods: Heifers and buffaloes with 70 days of pregnancy were inoculatedwith 1 108 tachyzoites of N. caninum (inoculated groups; bovine n ¼9, buffaloes n ¼ 2) and PBS (control group; bovine n ¼ 2, buffaloes n ¼ 2).Nc-1 (n: 3), Nc-6 Argentina (n: 3) and Nc-Spain 7 (n: 3) strains were usedin heifers. In buffaloes only Nc-1 strain was used. Placenta and uterussamples were collected at time of necropsy (28 days after inoculation).These tissues were analysed by histopathology analysis, immunhistochemistry(IHC) for N. caninum, lectinhistochemistry (LHC), PCR andmicrosatellite genotyping.Results: Histopathology analyses showed differences among groups, andno compatible lesions were found in control groups. IHC and PCR test werepositives to N. caninum in the inoculated groups and negatives in controlgroups. Microsatellite genotyping assay determined that every group hadjust the specific strain inoculated. LHC results were different betweenspecies. Besides, inoculated groups change the carbohydrate expression.Conclusion: N. canimum inoculation changes the pattern of saccharidespresent in the placenta and uterus tissues both in cattle and in buffaloes.This variation could generate alterations in pregnancy in infected animals.