INVESTIGADORES
DI VIRGILIO Ana Laura
congresos y reuniones científicas
Título:
Transition metal complexes with 6-methoxyquinoline as antitumor agents. Biological studies on two and three-dimensional cell cultured models.
Autor/es:
CADAVID VARGAS J.F.; VILLA PEREZ, C.; RUIZ, M.C.; LEON, I.E; VALENCIA G.C.; SORIA D.B.; DI VIRGILIO A.L.; ETCHEVERRY S.B.
Reunión:
Congreso; LXI Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2016
Resumen:
Coordination complexes have been extensively studied asantitumor agents, offering a potential alternative for cancer treatment.oth, the ligand and the metal play an important role in thepharmacological properties of the complex. This study evaluatesthe effect of methoxyquinoline complexes ith copper, zinc andsilver as antitumor agents in monolayer and a multicellularspheroids models derived from A and cell lines.Ag, Cu and n complexes significantly reduced the cell viability, evaluated by the TT assay in the model C .,.,. , respectively, A C ., .,. , respectively. oreover, reactive oxygen species Slevel as determined using dihydrorhodamine as a fluorescentprobe. nly the Cu complex induced S production ,.reliminary studies on synergy of the complexes ere performedusing the edianrug Effect analysis for Cu and n complexes atfixed ratio concentrations , and . The affected fractionas measured after h of incubation. t could be observed that nand Cu complexes presented a synergic effect at the ratios testedith a C .n the other hand, spheroids ere cultured by theliquid overlay technique and ere treated ith the complexes forh. The spheroids ere treated ith the Cu and n complexes.Hoever, this model as more resistant and the concentrationstested ere raised to times the C to reach a significant viabilityreduction ,. orphological changes ere observed inthe hole range of concentrations using phase contrast microscopy.oreover, the spheroids treated ith lo doses ere capable toreattach on the surface. All together, these results suggest that thefour complexes shoed a differential effect on the cultured cells. nand Cu complexes shoed the higher cytotoxic activity in both and 3D models. These compounds are good potential candidatesfor alternative antitumor treatment alone or in combination.