. TNF RECEPTOR DEFICIENCY DECREASES NITRIC OXIDE PRODUCTION BY PERITONEAL MACROPHAGES AND DECREASES LOCAL INFLAMMATION DURING CUTENEOUS LEISHMANIASIS

CARGNELUTTI, DIEGO; MACKERN OBERTI, JUAN PABLO; GERMANÓ, MJ

Mendoza

Congreso; Juan Pablo Mackern-Oberti; 2016

SBC

Leishmaniasis caused by Leishmania (Leishmania) amazonensis (LA) is a tropical infectious disease affecting different countries in South America. Due to LA infects macrophages, development of a Th1 immune response with production of TNF-α and IFN-γ is essential to eliminate this pathogens. The aim of our work was to evaluate the role of TNF receptor p55 subunit (TNF-R) in IFN-γ-induced nitric oxide (NO) production by peritoneal macrophages and their impact in cutaneous leishmaniasis. To this end we obtained peritoneal macrophages from TNF-R KO and WT mice and cultured for 24 hs with LPS (1µg/ml) and IFN-γ (10 UI/ml). NO production was determined by the Griess Reaction. Cutaneous leishmaniasis were performed by the infection of 1x105 LA promastigotes at mice left hinds pads. After 7 weeks, cutaneous lesions were surgery obtained, minced, stained with CD45 and analyzed by flow cytometry. We found that TNF-R KO macrophages showed a decreased production of NO after LPS+IFNγ compared to WT (WT,untreated UT, 2,1±0,9µM;WT,LPS+IFNγ,19,0±1,5µM;TNF-R KO,UT 1,1±0,5 µM;TNF-R KO, LPS+IFNγ, 3,±0,5 µM; p