INVESTIGADORES
LAINO Aldana
congresos y reuniones científicas
Título:
Study of lipid transport system in South American tarantula Grammostola rosea
Autor/es:
CUNNINGHAM MÓNICA; LAINO ALDANA; SUAREZ GUSTAVO; LINO AGUSTINA; GARCIA FERNANDO
Lugar:
Puerto varas
Reunión:
Congreso; XII PABMB Congress; 2013
Resumen:
Lipids due to its hydrophobic nature are transported in the
hemolymph mainly by lipoproteic fractions . In the present
work we studied the components of lipid transport of the
spider Grammosto/a rosea. Through ultracentrifugation
two lipoproteic fractions are isolated, one of high density
named Gr-HDL (o = 1.13-1.15 g/mi) and the other of very
high density called Gr-VHDL (o = 1.23-1.25 g/mi). GrVHDL
in hemolymph is majority in relation to Gr-HDL. In
this sense Gr-VHDL fraction has 98.6% of hemolymphatic
proteins, transporting 89.3% of lipids circulating in
hemolymph. Both lipoproteic fractions possess
phospholipids such as majority lipids and 18:1,16:0, 18:2
and 18:0 as the major fatty acids. Through in vitro assays
with different 14C_lipid, it was observed that Gr-VHDL takes
up mainly free fatty acids and triacylglycerols unlike that
observed for Gr-HDL in relation to phosphatidylcholine.
Through electrophoresis it was observed that Gr-VHDL
has three apolipoproteins: a predominant band of 68 kDa
and two others of 99 and 121 kDa. Gr-HDL displayed a
predominant band of 93kDa and other minoritary of 249
kDa.
In conclusion, this study reports lipid and proteic
characterization of the components of the lipid transport
system in tarantula, using G.rosea as a model. The role of
each protein in relation to lipid uptake is sustained by in
vitro assays.
This work was supported by grants from: CONICET-PIP
no. 075 and PICT no. 2010-1270, Argentina.