Cofilin-1 and associated genes relationship with melanoma radioresistance

GARCÍA, FRANCISCO M.; BRACALENTE, CANDELARIA; ZUCCATO, CAMILA; MILLER, TATIANA; BIOLATTI, VANESA; MOLINARI, BEATRIZ; KLAMT, FABIO; CASTRO, MAURO; IBAÑEZ, IRENE; DURÁN, HEBE

Mar del Plata

Congreso; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2016

Sociedad Argentina de Investigación Clínica

Melanoma is highly metastatic and in advanced stages resistant to chemo- and radiotherapies. Metastasis and resistance to therapies are decisive on the clinical outcome of patients. Detection of proteins involved in both processes could definepredictive andprognostic molecular markers that could be valuable to establish the therapeutic strategy and develop new therapies. One of the most relevant protein in cytoskeletal regulation is cofilin-1 (CFL1), which plays a central role in migration. CFL1 increased levels have been associated with aggressiveness in various cancers. Furthermore, it has been suggested as a marker of invasion and predictor of resistance to chemotherapy. We studied the relationship betweenexpression levels of proteins associated with regulation of the actin cytoskeleton and migration and metastatic phenotype, in a melanoma model (A375-G10: metastatic, A375-A7: non metastatic, A375-PCDNA3: control, all derived from A375). In this work CFL1 wasvalued as a predictive marker of radioresistance in melanoma radiotherapy. Firstof all, we determined radiosensivity by clonogenic assay, in which we showed A375-G10 cells were significantly more radioresistant than control and A375-A7 cells (p