Structural and functional characterization of human heteromeric 5-HT3 receptors

CORRADI J.; BOUZAT, C .

San Miguel de Tucuman

Congreso; IIILAFeBS, IX Iberoamerican Congress of Biophysics and XLV SAB Meeting; 2016

5-HT3 receptors are members of the Cys-loop receptor family that mediate fast excitatory transmission in central and peripheral nervous system. Genes for five different subunits (A-E) have been identified in humans, and most of the subunits have multimple isoforms. The A subunit is capable of forming functional homomeric (5-HT3A), or heteromeric receptors with the B subunit (S-HT3AB). Here we combine single-channel and macroscopic current recordings to determine if other 5-HT3 subunits, Br1, Br2, C, D and E(B-E), can combine with the A subunit to form heteromeric receports. After co-expression of the A subunit with each the tested subunits, single-channel events with different conductance and kinetic properties with respect to those of 5-HT3 receports were detected, except for the Br2 subunit. These results indicate that B-E subunits can assemble into functional heteromeric receptors with the A subunit. From the corresponding recordings, the analysis of the single-channel amplitude of the opening events suggests a possible stoichiometry for each heteromeric receptor.