The microencapsulation of the flavonoid genistein with soluble chitosan allows efficient release in colon and attenuation of oxidative stress during experimental colitis.

VANDEN BRABER, NOELIA; IVANNA NOVOTNY NUÑEZ; LUCIANA PAOLA BOHL; CARINA PORPORATTO; MARIANA A. MONTENEGRO; SILVIA CORREA

Mar del Plata

Congreso; LXI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2016

SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA

enistein 􀀊􀀩􀀋 is a flavonoid 􀁙ith antioxidant, anti􀀏inflammatoryand anti􀀏tumoral properties 􀁙hose absorption occurs in the stomachand small intestine 􀁙ith significant loss of the bioactivity. Thehypothesis of the present 􀁙or􀁍 􀁙as that upon microencapsulation,􀀩 could modulate the inflammatory process in the classiccolitis model. Colitis 􀁙as induced by administration of 􀀕􀀇 􀀊􀁙 /v􀀋 dextran sulfate sodium in drin􀁍ing 􀁙ater for 􀀗 days. 􀀩 􀁙asmicroencapsulated 􀀊􀀯C􀀩􀀋 in 􀁙ater soluble chitosan 􀀊􀀹SCh􀀋 byspray􀀏drying. Female C􀀗􀀙􀀤L/􀀘 mice 􀁙ere divided into 􀀗 groups􀀜Control, Colitis, Colitis􀀍􀀩, Colitis􀀍􀀯C􀀩 and Colitis􀀍􀀹SCh. 􀀩 􀀊􀀒.􀀒􀀚mg per day􀀋, 􀀯C􀀩 􀀊􀀕.􀀓􀀗 mg per day􀀋 and 􀀹SCh 􀀊􀀕.􀀒􀀙 mg perday􀀋 􀁙ere administered orally for 􀀙 days starting at the beginningof colitis induction and animals 􀁙ere clinically evaluated during􀀓􀀒 days. The disease activity index 􀀊􀀦A􀀫􀀋 􀁙as calculated through􀁙eight loss, consistency and bleeding stool. Colon 􀁙as isolatedand processed for pro and anti􀀏inflammatory cyto􀁍ine analysisand superoxide dismutase 􀀊S􀀱􀀦􀀋, catalase and myeloperoxidase􀀊􀀯􀀲􀀱􀀋 activities. Statistical analyses 􀁙ere performed by A􀀰􀀱􀀸A/Tu􀁍ey. 􀀫n 􀀯C􀀩 treated animals, the 􀀦A􀀫 􀁙as 􀀙􀀒􀀇 lo􀁙er than inColitis group 􀀊p􀀞􀀒.􀀒􀀗􀀋 􀁙hile Colitis􀀍􀀩 did not sho􀁙 significantimprovements. Levels of 􀀫L􀀏􀀘, T􀀰F􀀏α and MCP-1 decreased􀁙ith 􀀯C􀀩 administration 􀀊p􀀞􀀒.􀀒􀀗􀀋 􀁙hile a significant incrementof 􀀫L􀀏􀀓􀀒 􀁙as found. Colitis􀀍􀀹SCh group sho􀁙ed no remar􀁍ableclinical recovery although a mar􀁍ed decrease in pro􀀏inflammatorycyto􀁍ines and oxidative stress 􀁙as observed. S􀀱􀀦, catalase and􀀯􀀲􀀱 activities in Colitis􀀍􀀯C􀀩 group 􀁙ere comparable to Control.Together, the microencapsulation strategy allo􀁙ed the delivery ofthe bioactive flavonoid to the colon 􀁙ith an effective attenuation ofthe inflammation as 􀁙ell as the recovery of the oxidative balance.