analysis of free MC-LR levels by HLPC/MS in six tissues of mice orally exposed to the toxin

DANIELA SEDAN; GIANNUZZI L; ANDRINOLO, D

Congreso; 17th International Conference on Harmful Algae.; 2016

Oral route is one of the main routes of exposure to MC-LR, mainly through ingestion of contaminated water with this toxin.Although, toxicokinetics of MCs has been insufficiently discussed in the literature. The aim of this study was to evaluate thelevels of MC-LR present in the main body compartments in mice orally exposed to repeated doses of MC-LR.One group of N: NIH Swiss mice was treated by gavage with one dose of 250 μgMC-LR/kg and 30 '', 1 hr, 3hrs, 12hrs and 24hrs after the administration blood, liver, intestine, lung, kidney and heart samples were taken. Another group of mice wastreated by gavage with 4 doses of 250 μgMC-LR/kg administered every 48 hours. The same tissues samples were taken at24 and 48 hours post administration. Heart MC-LR levels were not detected in all experiments developed. The tissues mainlyinvolved in the uptake (intestine) and detoxification (liver and kidney) of MC-LR had equivalents toxin profile vs. time. Thesame occurred with toxins profile in blood and lung. Free MC-LR evidenced a different dynamic respect to the total toxin`sdynamic, mainly in the liver where showed no bioaccumulation of free MC-LR. The differences in processes and tissues thatMC-LR has access in oral administration respect of i.p. administration could generate the different dynamics observed.Furthermore animals have shown a physiological adaptation to successive doses of MC-LR that allowed them to have lowlevels of free MC-LR in target organs such as liver.