Titanium Concentrations in gingival Crevice Fluid and Saliva of Subjects with Dental Implants

M.S. GRENÓN; H. J. SÁNCHEZ; J. ABRAHAM; C. A. PÉREZ

Tucuman, Argentina

Conferencia; SAIO XXXIX; 2006

SAIO, Argentina

<!-- /* Font Definitions */ @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-1610611985 1107304683 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman","serif"; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-US;} .MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:10.0pt; mso-ansi-font-size:10.0pt; mso-bidi-font-size:10.0pt;} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Introduction. The elemental composition of gingival crevice fluid is important for understanding the negative role of periodontal diseases. Saliva has a significant participation in biofilm development and tartar formation; it also influences gingival illnesses through salival flux and its composition. Nowadays, the use of titanium dental implants is a common technique for dental restitution; nevertheless, the metabolic behavior of free titanium ions is not well understood yet, nor normal concentrations of this element in different organs and fluids are well known. The total-reflection x-ray fluorescence technique using synchrotron radiation (SR-TXRF) is an appropriate method to measure trace elements in liquid micro-samples. The aim of this work is to analyze the titanium concentration levels in oral fluids. Materials and Methods. Ten samples of gingival crevice fluid and saliva of patients with dental implants and twenty similar samples of a control group were collected by means of calibrated µ-capillaries. The gingival crevice fluids of the “implant” group were taken from a six-month old implant element or older. The samples were measured with the SR-TXRF technique and the spectra were analyzed with specific programs for spectrum analysis and quantitative determinations (AXIL). Results. The results show statistically significant differences of titanium concentrations for gingival crevice fluid in the “implant” group (22±7 µg/ml) with respect to the “control” group (1.3±0.4). Other elements also present different behaviors. No statistically significant variations of titanium concentrations in saliva were observed for both groups. Conclusions. Our results indicate that the presence of dental implants changes the concentrations of titanium and other elements in gingival crevice fluids but not in saliva.