A Comparison Between Elemental and Structural Analyses of Dental Tissues and Fluids using XRF Spectroscopy with SR and µ-IR Spectroscopy

H. J. SÁNCHEZ; M.S. GRENÓN; J. ABRAHAM; C. A. PÉREZ; C. MARCELLI; M. PICCININI; M. CESTELLI; M.C. VALENTINUZZI

Campinas, Brasil

Congreso; RAU 17; 2007

Laboratorio Nacional de Luz Sincrotrón

It has been well demonstrated that the di®erent oral tissues, including dental cal-culus and °uids are excellent descriptors and sentinels of, not only oral diseases,but also many other general diseases from cancer to osteoporosis or diabetes.Human dental calculus is a result of dental plaque calci¯cation. Calculus iscomposed by several calcium phosphate phases containing a number of elements atvery low concentration. When it ¯rst appears on the surface of dental pieces, theprimary phases su®er gradual crystalline-structure changes. The formation processof calculus is not well understood yet.It has been suggested that saliva and gingival °uid contains locally-derived andsystemically-derived markers of periodontal disease and, through this, the basis ofa speci¯c diagnostic tests for many other diseases.In previous works, conventional and microXRF analyses with synchrotron ra-diation were carried out on calculus and dental tissues in order to determine traceconcentrations giving insights into the growing process and their relation with sev-eral diseases. In addition, using SR-TXRF, traces and ultra traces were studiedin saliva and gingival °uids to determine several chemical indicators of developingperiodontal disease by comparison of elemental concentrations between two groupsof individuals, systemically healthy and periodontal-care required patients.Recently, similar samples of dental calculus, tooth tissues, saliva and gingival°uids were studied using micro-IR spectroscopy which provided information aboutstructures and phases in those samples.In this work we present a compilation of the data collected after x-ray spec-troscopy studies using synchrotron radiation and using micro-IR spectroscopy. Inaddition we present a comparison of common and comparable results obtained withboth techniques and the future perspectives of linked experiments.