Concentración de Minerales en Fluído gingival y Saliva de Pacientes Periodontales Fumadores y no Fumadores

M.S. GRENÓN; J. ABRAHAM; H. J. SÁNCHEZ; C. A. PÉREZ; A. BONO

Mar del Plata (ARG)

Congreso; , I Reunión Federación Latinoamericana, International Dental Research; 2005

International Dental Research

This work presents a study of elemental composition (major, minor and trace elements) of oralfluids such as saliva and gingival fluid, and their relation with smoking. Spectrochemical analyzeswere accomplished by the total-reflection x-ray fluorescence method using synchrotron radiation. Themain topic under investigation is the effect of chemical factors in the oral cavity which can influencethe development of gingival diseases.It is a well-known fact that the elemental composition of oral fluids is affected by the buccalbacterial flora and glandular activity. These changes of composition in saliva and gingival fluids werestudied in 20 patients that declared to be regular smokers. A similar number of non-smoker patientswere also studied. In the overall set of subjects, some of them presented different degrees of gingivaldisease while the rest were healthy. The analysis of some elements and their correlation with smokingcan be used to predict the development of some oral affections such as gingival illness.The samples were collected from adult patients in a dental office and deposited on reflectorscarriers for analysis. They were dried under lamp. A internal standard was added to the samples inorder to perform quantitative analysis. The experiments were carried out in the XRF beamline of theLNLS using the TXRF configuration and white beam.Data were analyzed with the usual programs for spectrum analysis. Quantification was attainedby using the sensitivity method with internal standard. The measured composition of oral fluids for thecases of smoker patients shows a significant increase of concentrations of certain elements and thepresence of other elements not detected in non-smoker subjects. Taking into account that some authorshave reported that unusual concentrations of elements like Ca, P and, in general, high-Z componentsrepresents a risk factor for the development of gingival illness, the results obtained in this work arebeen applied to establish the most significant correlations which will indicated specific effects on theoral diseases.