INVESTIGADORES
BLANCO Flavio Antonio
congresos y reuniones científicas
Título:
NF-YC1, a transcription factor required for nodule organogenesis, interacts with a putative protein kinase
Autor/es:
CLÚA, JOAQUÍN; RÍPODAS, CAROLINA; BATTAGLIA, MARINA; ZANETTI, MARÍA EUGENIA; BLANCO, FLAVIO
Lugar:
Portland
Reunión:
Congreso; Plant Biology 2014; 2014
Institución organizadora:
ASPB
Resumen:
Common bean (Phaseolus
vulgaris) establishes a nitrogen fixing association with its partner Rhizobium
etli. In this interaction, host-dependant competitiveness has been observed,
in which accessions from the Mesoamerican region are more efficient and
preferentially nodulated by strains that are predominant in the same
geographical region. A C subunit of the heterotrimeric nuclear factor Y (NF-Y), named NF-YC1, was
identified as a gene required for nodule organogenesis and bacterial infection that
contributes to this preferential association. In order to identify proteins
that can physically interact with NF-YC1, a yeast two hybrid screening was
performed using NF-YC1 as bait and a cDNA library from root tissue inoculated
with R. etli as prey. A
total of eight clones that potentially interacts with NF-YC1 were
isolated. Among them, one encodes a protein
kinase (PK) with a putative transmembrane domain at the N-terminus. The
bioinformatic analysis of the kinase domain indicates that it lacks two of the
three conserved residues required for catalytic activity. Interaction of NF-YC1
with this PK has been confirmed by retransformation of yeast and in planta by bimolecular fluorescent
complementation assays in Agrobacterium-infiltrated Nicotiana benthamiana
leaves. Expression of a translational fusion PK-GFP suggests that it
localizes to the cytoplasm and the plasma membrane. The function of this gene in
nodulation efficiency and bacterial infection is being evaluated both by RNAi
mediated-posttranscriptional gene silencing and ectopic expression in common
bean roots. This study will contribute to elucidate the signal transduction
pathway specifically activated in Mesoamerican common bean in response to its
cognate R. etli strain.