INVESTIGADORES
BLANCO Flavio Antonio
congresos y reuniones científicas
Título:
The monomeric GTPase ARFA1a locates to large moving vesicles and partially co-localize with RabA2
Autor/es:
RIVERO, CLAUDIO; TRAUBENIK, SOLEDAD; ZANETTI, MARÍA EUGENIA; BLANCO, FLAVIO; DALLA VIA, VIRGINIA
Reunión:
Congreso; XV Congreso Latinoamericano XXX Reunión Argentina de Fisiología Vegetal; 2014
Resumen:
Soil bacteria known as
rhizobia associate with legume
plants, resulting in the formation of nitrogen-fixing nodules in the root. Bacteria
are internalized through a tubular structure, the infection thread, which is
formed by an inversion of polar growth of root hairs. Formation of infectio
structures requires an active vesicle trafficking to transport membrane and
cell wall material. Monomeric GTPases participates in all steps of vesicle
transport across the cytosqueleton. We have previously shown that RabA2, a
monomeric Rab GTPase participates on polar growth root events and rhizobial
infection. ARFA1a was identified as a
gene co-regulated with RabA2. Localization experiments showed that ArfA1a-RFP
localized on relatively large punctuate structures of 2-3 µm located in the cytoplasm
and the membrane. These moving structures resemble the subcellular localization
previously described for ArfA1c in Arabidopsis
thaliana. Moreover, localization was unaffected after rhizobia inoculation
and after BFA treatment. Agroinfiltracion of Nicotiana benthamina leaves showed that GFP-RabA2 partially co-localized
with ArfA1a-RFP . Co-expression of ArfA1a-GFP with markers of known subcelullar
organelles will help to elucidate the identity of structures labeled by ArfA1a.