INVESTIGADORES
BLANCO Flavio Antonio
congresos y reuniones científicas
Título:
Dynamics of cytoplasmic mRNAs at early stages of the nitrogen fixing symbiosis between the model legume Medicago truncatula and Sinorhizobium meliloti
Autor/es:
LANCIA, MARCOS; REYNOSO, MAURICIO; BLANCO, FLAVIO; ZANETTI, MARÍA EUGENIA
Reunión:
Congreso; . XV Congreso Latinoamericano XXX Reunión Argentina de Fisiología Vegetal; 2014
Resumen:
Eukaryotic mRNAs associate to RNA-binding proteins that influence their stability,
transport, cellular localization and translatability. In the cytoplasm, mRNAs
can be translated into polysomes, targeted for degradation into P-bodies or
stored in cytoplasmic granules. We have genome-wide characterized changes
in the association of mRNAs to polysomes in roots of the model legume Medicago truncatula at early stages of the
symbiotic interaction with Sinorhizobium meliloti
using Translating Ribosomes Affinity Purification (TRAP) combined with RNA -Seq
technology. This analysis identified a number of mRNAs that do not change in total
abundance, but are significantly up- or down-regulated at the level of
association with polysomes. The first category includes genes that have
shown to play essential roles in nodulation, such as those encoding a pectate
lyase and a SINA family member. The second category contains mRNAs that
dissociate from polysomes upon rhizobial infection and is represented by NCR
secreted peptides, receptor-like kinases and SAUR-auxin responsive proteins.
Differential recruitment of these mRNAs to polysomes is being validated by
RT-qPCR in the whole root and in specific tissue types. The dynamic partitioning of mRNAs between
polysomes, P-bodies and cytoplasmic
granules is currently being evaluated by
affinity purification of these RNA-protein complexes.