INVESTIGADORES
BLANCO Flavio Antonio
congresos y reuniones científicas
Título:
Small RNA involved in the preferential symbiotic association between Phaseolus vulgaris and Rhizobium etli.
Autor/es:
CASTAINGTS, MELISSE; DALLA VIA, VIRGINIA; AGUILAR, O. MARIO; BLANCO, FLAVIO; ZANETTI, MARÍA EUGENIA
Lugar:
Mar del Plata
Reunión:
Congreso; . XV Congreso Latinoamericano XXX Reunión Argentina de Fisiología Vegetal.; 2014
Resumen:
Common bean (Phaseolus vulgaris) establishes a
symbiotic interaction with Rhizobium etli,
resulting in the formation of root nitrogen-fixing nodules. Interestingly,
beans from the Mesoamerican center of genetic diversification are able to
discriminate different strain of R. etli
which are more efficient in the nodulation process. Our group has identified several
protein-coding genes involved in this preferential interaction. Recently, we
focused our studies on the identification of small RNAs (sRNAs), including both
microRNAs (miRNAs) and small interference RNAs (siRNAs), that might participate
in the strain preference observed in Mesoamerican beans. We generated sRNAs
libraries from non-inoculated roots or from roots inoculated with strains of R. etli with different nodulation
efficiency. Sequencing of the libraries using Illumina technology (1x50bp)
yielded an average of 28 millions reads per library with primary lengths of 21
and 24 nucleotides. Reads were mapped to the P. vulgaris reference genome, revealing that most of them mapped to
intergenic regions. We are analyzing changes in sRNAs abundance in each tested condition
by using bioinformatics tools. This approach will allow us to identify and
select new sRNAs with potential roles in this preferentially symbiosis, which
will be further characterized by
functional genetic.