INVESTIGADORES
BLANCO Flavio Antonio
congresos y reuniones científicas
Título:
Dissecting the molecular response of common bean to Rhizobium etli with next-generation sequencing technologies (NGST).
Autor/es:
DALLA VIA, VIRGINIA; NARDUZZI, CANDELA; LANCIA, MARCOS; AGUILAR, MARIO; ZANETTI, MARÍA EUGENIA; BLANCO, FLAVIO
Lugar:
Chascomús
Reunión:
Congreso; XV Jornadas anuales de la Sociedad Argentina de Biología; 2013
Resumen:
Legume plants establish a mutualistic symbiosis with soil bacteria
that convert N2 to forms that can be directly incorporated into the
metabolism. Understanding the molecular basis of this process is key to
alleviate the use of chemical fertilizers, preventing the environmental
pollution associated to modern agricultural practices. In this work, we used
next generation sequencing technologies to unravel two aspects of the symbiotic
interaction, the root transcriptional response modulated by bacterial signals
and the molecular basis of the selection of the most efficient strains by the
plant. To achieve the first objective, mRNA from root tissue of common bean
plants inoculated with the wild type
strain of R. etli or with mutant
strains that are impaired in the synthesis of the Nod Factor, the
exopolysaccharide and, the lipopolysaccharide I
were used to construct Illumina libraries that have been sequenced. For
the second objective, we took advantage of the evolution of common bean in two
centers of genetic diversification, the Mesoamerican and the Andean regions,
which resulted in the capability of Mesoamerican accessions to preferentially
associate with R. etli strains that are prevalent in Mesoamerican soils.
This specific association is correlated with a more efficient nodulation and
nitrogen fixation. Illumina libraries were prepared to compare the
transcriptome of roots inoculated with a more efficient strain with that of
roots inoculated with the less efficient one. Expression of selected genes
was confirmed by RT-qPCR. Our current progress in NGST analysis, as well as
further implication of our findings, will be discussed.