Stereoselective bioreduction of bulky-bulky ketones by the wild yeast Rhodotorula sp. LSL

AGUIRRE PRANZONI C.; BISOGNO F.R; ORDEN A.A.; KURINA SANZ M.

Buzios

Simposio; VI Workshop on Biocatalysis and Biotransformations. 1° Simposio Latioamericano de Biocatálisis y Biotransformaciones (I SiLaByB); 2014

used to generate optically pure sec-alcohols, stereoselective reduction towards bulky aromatic substrates are still rare. Most of the selective ADHs possessing a broad substrate spectrum resembles small bulky ketones, thus, the small substituent is limited to a sterically non demanding group such as methyl, ethyl, or chloromethyl. Only a few organisms or enzymes have been reported for the reduction of bulky-bulky ketones. Rhodotorula sp. LSL is a chemo extremophile wild microorganism isolated from a landfarming. We have already showed its ability for the stereoselective reduction of a series of non-sterically demanding ketones, and its use as a lyophilized catalyst in one pot chemoenzymatic procedures was optimized. Since to generate of stereogenic centers at the endgame of the synthesis of pharmaceutically potential products is an ongoing hot topic, we chose ketotriazoles as substrates to identify the ability of this new catalyst for the reduction of bulky bulky ketones.