INVESTIGADORES
LIJAVETZKY Diego Claudio
congresos y reuniones científicas
Título:
Effect of elicitors on stilbene biosynthesis gene expression and resveratrol production in Monastrell grapevine cell cultures
Autor/es:
BELCHI-NAVARRO, S; ALMAGRO, L; LIJAVETZKY, D; BRU, R; PEDRENO, MA
Lugar:
Tampere, Finland
Reunión:
Congreso; XVI Congress of the Federation of European Societies of Plant Biology (FESPB); 2008
Resumen:
Vitaceae phytoalexins constitute a group of molecules belonging to the
stilbene family which are derivatives of resveratrol structure. Due to its
potential benefi ts on human health, resveratrol have become one of the
most thoroughly studied molecules, that is why Vitis vinifera cell cultures
have been used in several studies to investigate the factors involved in
the induction and regulation of stilbene biosynthesis. Using cyclodextrins
(CD) as elicitors on grapevine cell cultures, we have developed an
innovative procedure where high levels of this metabolite is accumulated
and easily recovered directly from the culture media without cell
biomass destruction. Moreover, methyljasmonate (MeJA), salicylic acid
and ethylene which are involved in defence responses, were added
to cell suspensions resulting in modifi ed resveratrol accumulation
positively or negatively. The effects of the different elicitor treatments
in resveratrol production were analysed reaching a maximum accumulation
after 120 h (1400 mmole/gDW) when cells were simultaneously
elicited with CD and MeJA. We also analysed the relationship
between levels of resveratrol and the expression of related biosynthetic
genes by performing real-time qRT-PCR of stilbene biosynthesis genes.
Acknowledgements L.A. and S.B.N. hold grants from the Fundacion
Seneca. This work has been partially supported by the MEC and FEDER
(BIO2005-00332) and by the CARM (BIO-BVA 07/01-0003).