ANTIFUNGAL ACTIVITY OF SOLIDAGO CHILENSIS AGAINST POSTHARVEST PHYTOPATHOGENIC FUNGI

DI LIBERTO, MELINA; SVETAZ, LAURA; DERITA, MARCOS

Rosario

Congreso; 4ta Reunión Internacional de Ciencias Farmacéuticas; 2016

Facultad de Ciencias Bioquímicas y Farmacéuticas (UNR) y Facultad de Ciencias Químicas (UNC)

The continuous and excessive use of synthetics fungicides in postharvested fruits causes dangerous consequences to the environment and the human health. In this context, exploring natural sources based on native plant extracts, turns out to be of great economic and social importance. Solidago chilensis Meyen, an Argentinean native species with previously reported human antifungal properties, was evaluated against two peach phytopathogenic fungi: Monilinia fructicola and Rhizopus stolonifer.S. chilensis, was collected during the flowering season (March 2014) in Santa Fe province (Argentina). Powered aerial parts were successively macerated (3×24 h each) with petroleum ether (H), ethyl acetate (EtOAc) and ethanol (EtOH) with mechanical stirring to obtain the corresponding extracts, after filtration and evaporation. The essential oil (EO) was obtained from fresh aerial parts using a Clevenger device, according to the European?s Pharmacopea technique.For the antifungal evaluation, inocula of spore suspensions of M. fructicola (M.f.) and R. stolonifer (R.s.) were adjusted to 1 x104 Colony Forming Units/mL. The Minimum Inhibitory Concentrations (MICs) were determined using broth microdilution techniques according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI) for filamentous fungi.The H and EtOAc extracts showed the highest potency (MICs = 62.5 and 250 µg/mL, respectively) against R.s and MICs = 125 µg/mL against M.f. EO presented moderate activity against M.f. (MIC = 250 µg/mL) and was hardly active against R.s. (MIC = 1000 µg/mL). EtOH extract resulted barely active against M.f. (MIC = 1000 µg/mL) and inactive against R.s. (MIC > 1000 µg/mL).