INQUISAL   20936
INSTITUTO DE QUIMICA DE SAN LUIS "DR. ROBERTO ANTONIO OLSINA"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Monitoring of heavy metals in wheat and sunflowers by micellar electrokinetic chromatography with indirect detection
Autor/es:
PATRICIA WANDA STEGE; LORENA L. SOMBRA; GERMÁN ALEJANDRO MESSINA; JOSE GASQUEZ; LUIS DANTE MARTINEZ; ROBERTO A. OLSINA
Lugar:
Puerto Vallarta, Mexico.
Reunión:
Congreso; LACE; 2008
Resumen:
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Human activity such as industrial
development, urbanization and intensive agriculture, has led to extensive
environmental problems. Since heavy metals are mobile and easily absorbed by
plants in the environment, they are transmitted to the human body through
nourishment [1, 2]. Metal-accumulating
plants, such Triticum species and Helianthus annuus L., can accumulate high
concentrations of heavy metals in both leaves and roots from polluted soil and
waters [3].
For the determination of these elements we used CZE
with indirect detection. This technique require the dissolution of the sample,
with this aim three digestion procedures were applied. The digestion procedures
tested were: Procedure 1: batch using a mixture of HNO3+HCl;
Procedure 2: microwave (MW)-assisted acid treatment with an acid mixture of HNO3+HCl
and Procedure 3: microwave (MW)-assisted acid treatment with H2O2.
The samples obtained by the diferent proscedures were inyected to the CE. The
detection was performed at 214 nm; the voltage was set at 25 kV. Samples were
pressure-injected at the cathodic side at 0.5 psi for 10 seconds. The
background electrolyte (BGE) used consisted of 6 mM imidazole, pH 4.0 adjusted
with 1 M acetic acid. The best results were acquired when the digestion
procedure 1 was applied.
The proposed method allows the rapid determination of
heavy metals in natural products. It was recently demonstrated that MW-assisted
acid treatment with H2O2 could be used satisfactorily for
sample clean-up technique prior to CE determination of various metals species. This
methods have a great potential to be
applied in phytoremediation in which the use of green plants help to remove
contaminants from soil and groundwater.