INSIBIO   05451
INSTITUTO SUPERIOR DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Bacterial composition in high altitude Andean Wetlands freshwater lakes, as determined by denaturing gradient gel electrophoresis (DGGE).
Autor/es:
M. C. ESTÉVEZ, O.F. ORDOÑEZ, M. R. FLORES, M. E. FARÍAS.
Lugar:
Cairns, Australia
Reunión:
Simposio; 12th International Symposium on Microbial Ecology; 2008
Institución organizadora:
International Society for Microbial Ecology (ISME)
Resumen:
The high altitude Andean Wetlands presents unique features for the study of
bacterial diversity. In this area are freswater lakes located more than 4,200
meters above sea level with extreme environmental conditions: high salinity,
high incidence of UV, low phosphorus content, high content of heavy metals,
etc. The aim of this work was study the bacterial composition of 6 lakes by
denaturing gradient gel electrophoresis (DGGE). ). Four of the lakes that make
up the Salar de la laguna Verde (L. Azul, L. Verde, L. Negra and L. Aparejos),
lake Vilama and lake Catal corresponding to Salar del Hombre Muerto were
sampled during the summers of 2006 and 2007.
Bacterioplankton community composition in all of the lakes was represented
mainly by Gamma-proteobacteria (Halomonas sp., Marinomonas sp,
Stenotrophomonas sp., Xanthomonas sp.) Alpha-proteobacteria (Roseobacter
Stenotrophomonas sp., Xanthomonas sp.) Alpha-proteobacteria (Roseobacter
Stenotrophomonas sp., Xanthomonas sp.) Alpha-proteobacteria (Roseobacter
Stenotrophomonas sp., Xanthomonas sp.) Alpha-proteobacteria (Roseobacter
Halomonas sp., Marinomonas sp,
Stenotrophomonas sp., Xanthomonas sp.) Alpha-proteobacteria (RoseobacterRoseobacter
sp.), Beta proteobacterias (Aquabacetrium commune sp.), HGC,
(Staphylococcus sp., Agrococcus jenensis and uncultured bacterium) and CFB
(Uncultured Bacteroidetes). In L. Negra and L. Aparejos there is a
predominance of Gamaproteobacterias (60%), while in the Azul and Catal,
OTUS found were represented equally by all filos. Firmicutes was the other filo
predominant, with 20% en Azul, Vilama, Catal and Aparejos. Bacteroidetes is
found in all lakes sampled at a rate that varies from 15 to 30%.
The most of the bands shared among lakes. There was not appear bands
exclusively in specific lakes. Interestingly, many of the sequences showed high
identity with oligotrophic and extremophile bacteria collected in GenBank, which
suggests bacterial assemblages well adapted to extreme environmental
conditions.
(Uncultured Bacteroidetes). In L. Negra and L. Aparejos there is a
predominance of Gamaproteobacterias (60%), while in the Azul and Catal,
OTUS found were represented equally by all filos. Firmicutes was the other filo
predominant, with 20% en Azul, Vilama, Catal and Aparejos. Bacteroidetes is
found in all lakes sampled at a rate that varies from 15 to 30%.
The most of the bands shared among lakes. There was not appear bands
exclusively in specific lakes. Interestingly, many of the sequences showed high
identity with oligotrophic and extremophile bacteria collected in GenBank, which
suggests bacterial assemblages well adapted to extreme environmental
conditions.
(Uncultured Bacteroidetes). In L. Negra and L. Aparejos there is a
predominance of Gamaproteobacterias (60%), while in the Azul and Catal,
OTUS found were represented equally by all filos. Firmicutes was the other filo
predominant, with 20% en Azul, Vilama, Catal and Aparejos. Bacteroidetes is
found in all lakes sampled at a rate that varies from 15 to 30%.
The most of the bands shared among lakes. There was not appear bands
exclusively in specific lakes. Interestingly, many of the sequences showed high
identity with oligotrophic and extremophile bacteria collected in GenBank, which
suggests bacterial assemblages well adapted to extreme environmental
conditions.
(Uncultured Bacteroidetes). In L. Negra and L. Aparejos there is a
predominance of Gamaproteobacterias (60%), while in the Azul and Catal,
OTUS found were represented equally by all filos. Firmicutes was the other filo
predominant, with 20% en Azul, Vilama, Catal and Aparejos. Bacteroidetes is
found in all lakes sampled at a rate that varies from 15 to 30%.
The most of the bands shared among lakes. There was not appear bands
exclusively in specific lakes. Interestingly, many of the sequences showed high
identity with oligotrophic and extremophile bacteria collected in GenBank, which
suggests bacterial assemblages well adapted to extreme environmental
conditions.
(Staphylococcus sp., Agrococcus jenensis and uncultured bacterium) and CFB
(Uncultured Bacteroidetes). In L. Negra and L. Aparejos there is a
predominance of Gamaproteobacterias (60%), while in the Azul and Catal,
OTUS found were represented equally by all filos. Firmicutes was the other filo
predominant, with 20% en Azul, Vilama, Catal and Aparejos. Bacteroidetes is
found in all lakes sampled at a rate that varies from 15 to 30%.
The most of the bands shared among lakes. There was not appear bands
exclusively in specific lakes. Interestingly, many of the sequences showed high
identity with oligotrophic and extremophile bacteria collected in GenBank, which
suggests bacterial assemblages well adapted to extreme environmental
conditions.
(Uncultured Bacteroidetes). In L. Negra and L. Aparejos there is a
predominance of Gamaproteobacterias (60%), while in the Azul and Catal,
OTUS found were represented equally by all filos. Firmicutes was the other filo
predominant, with 20% en Azul, Vilama, Catal and Aparejos. Bacteroidetes is
found in all lakes sampled at a rate that varies from 15 to 30%.
The most of the bands shared among lakes. There was not appear bands
exclusively in specific lakes. Interestingly, many of the sequences showed high
identity with oligotrophic and extremophile bacteria collected in GenBank, which
suggests bacterial assemblages well adapted to extreme environmental
conditions.
(Uncultured Bacteroidetes). In L. Negra and L. Aparejos there is a
predominance of Gamaproteobacterias (60%), while in the Azul and Catal,
OTUS found were represented equally by all filos. Firmicutes was the other filo
predominant, with 20% en Azul, Vilama, Catal and Aparejos. Bacteroidetes is
found in all lakes sampled at a rate that varies from 15 to 30%.
The most of the bands shared among lakes. There was not appear bands
exclusively in specific lakes. Interestingly, many of the sequences showed high
identity with oligotrophic and extremophile bacteria collected in GenBank, which
suggests bacterial assemblages well adapted to extreme environmental
conditions.
(Uncultured Bacteroidetes). In L. Negra and L. Aparejos there is a
predominance of Gamaproteobacterias (60%), while in the Azul and Catal,
OTUS found were represented equally by all filos. Firmicutes was the other filo
predominant, with 20% en Azul, Vilama, Catal and Aparejos. Bacteroidetes is
found in all lakes sampled at a rate that varies from 15 to 30%.
The most of the bands shared among lakes. There was not appear bands
exclusively in specific lakes. Interestingly, many of the sequences showed high
identity with oligotrophic and extremophile bacteria collected in GenBank, which
suggests bacterial assemblages well adapted to extreme environmental
conditions.
(Staphylococcus sp., Agrococcus jenensis and uncultured bacterium) and CFB
(Uncultured Bacteroidetes). In L. Negra and L. Aparejos there is a
predominance of Gamaproteobacterias (60%), while in the Azul and Catal,
OTUS found were represented equally by all filos. Firmicutes was the other filo
predominant, with 20% en Azul, Vilama, Catal and Aparejos. Bacteroidetes is
found in all lakes sampled at a rate that varies from 15 to 30%.
The most of the bands shared among lakes. There was not appear bands
exclusively in specific lakes. Interestingly, many of the sequences showed high
identity with oligotrophic and extremophile bacteria collected in GenBank, which
suggests bacterial assemblages well adapted to extreme environmental
conditions.
(Uncultured Bacteroidetes). In L. Negra and L. Aparejos there is a
predominance of Gamaproteobacterias (60%), while in the Azul and Catal,
OTUS found were represented equally by all filos. Firmicutes was the other filo
predominant, with 20% en Azul, Vilama, Catal and Aparejos. Bacteroidetes is
found in all lakes sampled at a rate that varies from 15 to 30%.
The most of the bands shared among lakes. There was not appear bands
exclusively in specific lakes. Interestingly, many of the sequences showed high
identity with oligotrophic and extremophile bacteria collected in GenBank, which
suggests bacterial assemblages well adapted to extreme environmental
conditions.
(Uncultured Bacteroidetes). In L. Negra and L. Aparejos there is a
predominance of Gamaproteobacterias (60%), while in the Azul and Catal,
OTUS found were represented equally by all filos. Firmicutes was the other filo
predominant, with 20% en Azul, Vilama, Catal and Aparejos. Bacteroidetes is
found in all lakes sampled at a rate that varies from 15 to 30%.
The most of the bands shared among lakes. There was not appear bands
exclusively in specific lakes. Interestingly, many of the sequences showed high
identity with oligotrophic and extremophile bacteria collected in GenBank, which
suggests bacterial assemblages well adapted to extreme environmental
conditions.
(Uncultured Bacteroidetes). In L. Negra and L. Aparejos there is a
predominance of Gamaproteobacterias (60%), while in the Azul and Catal,
OTUS found were represented equally by all filos. Firmicutes was the other filo
predominant, with 20% en Azul, Vilama, Catal and Aparejos. Bacteroidetes is
found in all lakes sampled at a rate that varies from 15 to 30%.
The most of the bands shared among lakes. There was not appear bands
exclusively in specific lakes. Interestingly, many of the sequences showed high
identity with oligotrophic and extremophile bacteria collected in GenBank, which
suggests bacterial assemblages well adapted to extreme environmental
conditions.
(Staphylococcus sp., Agrococcus jenensis and uncultured bacterium) and CFB
(Uncultured Bacteroidetes). In L. Negra and L. Aparejos there is a
predominance of Gamaproteobacterias (60%), while in the Azul and Catal,
OTUS found were represented equally by all filos. Firmicutes was the other filo
predominant, with 20% en Azul, Vilama, Catal and Aparejos. Bacteroidetes is
found in all lakes sampled at a rate that varies from 15 to 30%.
The most of the bands shared among lakes. There was not appear bands
exclusively in specific lakes. Interestingly, many of the sequences showed high
identity with oligotrophic and extremophile bacteria collected in GenBank, which
suggests bacterial assemblages well adapted to extreme environmental
conditions.
(Uncultured Bacteroidetes). In L. Negra and L. Aparejos there is a
predominance of Gamaproteobacterias (60%), while in the Azul and Catal,
OTUS found were represented equally by all filos. Firmicutes was the other filo
predominant, with 20% en Azul, Vilama, Catal and Aparejos. Bacteroidetes is
found in all lakes sampled at a rate that varies from 15 to 30%.
The most of the bands shared among lakes. There was not appear bands
exclusively in specific lakes. Interestingly, many of the sequences showed high
identity with oligotrophic and extremophile bacteria collected in GenBank, which
suggests bacterial assemblages well adapted to extreme environmental
conditions.
(Uncultured Bacteroidetes). In L. Negra and L. Aparejos there is a
predominance of Gamaproteobacterias (60%), while in the Azul and Catal,
OTUS found were represented equally by all filos. Firmicutes was the other filo
predominant, with 20% en Azul, Vilama, Catal and Aparejos. Bacteroidetes is
found in all lakes sampled at a rate that varies from 15 to 30%.
The most of the bands shared among lakes. There was not appear bands
exclusively in specific lakes. Interestingly, many of the sequences showed high
identity with oligotrophic and extremophile bacteria collected in GenBank, which
suggests bacterial assemblages well adapted to extreme environmental
conditions.
(Uncultured Bacteroidetes). In L. Negra and L. Aparejos there is a
predominance of Gamaproteobacterias (60%), while in the Azul and Catal,
OTUS found were represented equally by all filos. Firmicutes was the other filo
predominant, with 20% en Azul, Vilama, Catal and Aparejos. Bacteroidetes is
found in all lakes sampled at a rate that varies from 15 to 30%.
The most of the bands shared among lakes. There was not appear bands
exclusively in specific lakes. Interestingly, many of the sequences showed high
identity with oligotrophic and extremophile bacteria collected in GenBank, which
suggests bacterial assemblages well adapted to extreme environmental
conditions.
(Aquabacetrium commune sp.), HGC,
(Staphylococcus sp., Agrococcus jenensis and uncultured bacterium) and CFB
(Uncultured Bacteroidetes). In L. Negra and L. Aparejos there is a
predominance of Gamaproteobacterias (60%), while in the Azul and Catal,
OTUS found were represented equally by all filos. Firmicutes was the other filo
predominant, with 20% en Azul, Vilama, Catal and Aparejos. Bacteroidetes is
found in all lakes sampled at a rate that varies from 15 to 30%.
The most of the bands shared among lakes. There was not appear bands
exclusively in specific lakes. Interestingly, many of the sequences showed high
identity with oligotrophic and extremophile bacteria collected in GenBank, which
suggests bacterial assemblages well adapted to extreme environmental
conditions.
(Uncultured Bacteroidetes). In L. Negra and L. Aparejos there is a
predominance of Gamaproteobacterias (60%), while in the Azul and Catal,
OTUS found were represented equally by all filos. Firmicutes was the other filo
predominant, with 20% en Azul, Vilama, Catal and Aparejos. Bacteroidetes is
found in all lakes sampled at a rate that varies from 15 to 30%.
The most of the bands shared among lakes. There was not appear bands
exclusively in specific lakes. Interestingly, many of the sequences showed high
identity with oligotrophic and extremophile bacteria collected in GenBank, which
suggests bacterial assemblages well adapted to extreme environmental
conditions.
(Uncultured Bacteroidetes). In L. Negra and L. Aparejos there is a
predominance of Gamaproteobacterias (60%), while in the Azul and Catal,
OTUS found were represented equally by all filos. Firmicutes was the other filo
predominant, with 20% en Azul, Vilama, Catal and Aparejos. Bacteroidetes is
found in all lakes sampled at a rate that varies from 15 to 30%.
The most of the bands shared among lakes. There was not appear bands
exclusively in specific lakes. Interestingly, many of the sequences showed high
identity with oligotrophic and extremophile bacteria collected in GenBank, which
suggests bacterial assemblages well adapted to extreme environmental
conditions.
(Uncultured Bacteroidetes). In L. Negra and L. Aparejos there is a
predominance of Gamaproteobacterias (60%), while in the Azul and Catal,
OTUS found were represented equally by all filos. Firmicutes was the other filo
predominant, with 20% en Azul, Vilama, Catal and Aparejos. Bacteroidetes is
found in all lakes sampled at a rate that varies from 15 to 30%.
The most of the bands shared among lakes. There was not appear bands
exclusively in specific lakes. Interestingly, many of the sequences showed high
identity with oligotrophic and extremophile bacteria collected in GenBank, which
suggests bacterial assemblages well adapted to extreme environmental
conditions.
Agrococcus jenensis and uncultured bacterium) and CFB
(Uncultured Bacteroidetes). In L. Negra and L. Aparejos there is a
predominance of Gamaproteobacterias (60%), while in the Azul and Catal,
OTUS found were represented equally by all filos. Firmicutes was the other filo
predominant, with 20% en Azul, Vilama, Catal and Aparejos. Bacteroidetes is
found in all lakes sampled at a rate that varies from 15 to 30%.
The most of the bands shared among lakes. There was not appear bands
exclusively in specific lakes. Interestingly, many of the sequences showed high
identity with oligotrophic and extremophile bacteria collected in GenBank, which
suggests bacterial assemblages well adapted to extreme environmental
conditions.