INSIBIO   05451
INSTITUTO SUPERIOR DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Incidence of UV-B artificial radiation on bacterial community composition, in two andean wetlands (4,400 m).
Autor/es:
M.R. FLORES, M.C. ESTÉVEZ, O.F. ORDÓÑEZ, AND M.E. FARÍAS.
Lugar:
Cairns, Australia
Reunión:
Simposio; 12th International Symposium on Microbial Ecology; 2008
Institución organizadora:
International Society for Microbial Ecology (ISME)
Resumen:
Laguna (L.) Negra and L. Verde are wetlands located in the northwest of Argentina
(4,400 meter above sea level) and exposed to high UV-B radiation and temperature
changes. The aim of this work was to study the effects of UV-B radiation on
bacterioplankton from these enviroments.
Superficial water samples from these wetlands were exposed to artificial UV-B
radiation (in vitro) during 24hs, with frecuency sampling of 1, 3, 6, 12 and 24hs.
Denaturing Gradient Gel Electrophoresis (DGGE) analisys, Cyclobutane Pyrimidine
Dimers (CPDs) accumulation and bacteria isolation at different media were performed.
Bacteria isolation was done by plating in two culture media: Luria Bertani (LB) and
Lagoon Media (LM). Among bacteria isolated the most resistant strains were different
species of Exiguobacterium spp. from L. Negra and Staphylococcus spp. (LM) and
Denaturing Gradient Gel Electrophoresis (DGGE) analisys, Cyclobutane Pyrimidine
Dimers (CPDs) accumulation and bacteria isolation at different media were performed.
Bacteria isolation was done by plating in two culture media: Luria Bertani (LB) and
Lagoon Media (LM). Among bacteria isolated the most resistant strains were different
species of Exiguobacterium spp. from L. Negra and Staphylococcus spp. (LM) and
in vitro) during 24hs, with frecuency sampling of 1, 3, 6, 12 and 24hs.
Denaturing Gradient Gel Electrophoresis (DGGE) analisys, Cyclobutane Pyrimidine
Dimers (CPDs) accumulation and bacteria isolation at different media were performed.
Bacteria isolation was done by plating in two culture media: Luria Bertani (LB) and
Lagoon Media (LM). Among bacteria isolated the most resistant strains were different
species of Exiguobacterium spp. from L. Negra and Staphylococcus spp. (LM) andExiguobacterium spp. from L. Negra and Staphylococcus spp. (LM) and
Stenotrophomonas maltophilia (LB) from L. Verde. UV-B radiaton resistance was
mainly observed in colored bateria. DGGE analices showed the precence of band that
presented high similitude with Gamma-proteobacteria, Alpha-proteobacteria and
Bacteroidetes in both wetlands. UV-B resistance associated to a specific phylogenetic
group was not detected in these environments. CPDs accumulation was observed, the
amounts increased from 0 to1280 CPD MB-1 in these wetlands after 6 hr of exposition,
and it was lower than biodosimeters (of calf thymus DNA), denoting efficient UV-B
resistant mechanisms.
According to the found results, it can be concluded that bacterial communities in the
studied environments are well adapted to high UV-B radiation there is a relationship
bewteen pigmentation and UV-B resistance.
and it was lower than biodosimeters (of calf thymus DNA), denoting efficient UV-B
resistant mechanisms.
According to the found results, it can be concluded that bacterial communities in the
studied environments are well adapted to high UV-B radiation there is a relationship
bewteen pigmentation and UV-B resistance.
mainly observed in colored bateria. DGGE analices showed the precence of band that
presented high similitude with Gamma-proteobacteria, Alpha-proteobacteria and
Bacteroidetes in both wetlands. UV-B resistance associated to a specific phylogenetic
group was not detected in these environments. CPDs accumulation was observed, the
amounts increased from 0 to1280 CPD MB-1 in these wetlands after 6 hr of exposition,
and it was lower than biodosimeters (of calf thymus DNA), denoting efficient UV-B
resistant mechanisms.
According to the found results, it can be concluded that bacterial communities in the
studied environments are well adapted to high UV-B radiation there is a relationship
bewteen pigmentation and UV-B resistance.
and it was lower than biodosimeters (of calf thymus DNA), denoting efficient UV-B
resistant mechanisms.
According to the found results, it can be concluded that bacterial communities in the
studied environments are well adapted to high UV-B radiation there is a relationship
bewteen pigmentation and UV-B resistance.
(LB) from L. Verde. UV-B radiaton resistance was
mainly observed in colored bateria. DGGE analices showed the precence of band that
presented high similitude with Gamma-proteobacteria, Alpha-proteobacteria and
Bacteroidetes in both wetlands. UV-B resistance associated to a specific phylogenetic
group was not detected in these environments. CPDs accumulation was observed, the
amounts increased from 0 to1280 CPD MB-1 in these wetlands after 6 hr of exposition,
and it was lower than biodosimeters (of calf thymus DNA), denoting efficient UV-B
resistant mechanisms.
According to the found results, it can be concluded that bacterial communities in the
studied environments are well adapted to high UV-B radiation there is a relationship
bewteen pigmentation and UV-B resistance.
and it was lower than biodosimeters (of calf thymus DNA), denoting efficient UV-B
resistant mechanisms.
According to the found results, it can be concluded that bacterial communities in the
studied environments are well adapted to high UV-B radiation there is a relationship
bewteen pigmentation and UV-B resistance.
-1 in these wetlands after 6 hr of exposition,
and it was lower than biodosimeters (of calf thymus DNA), denoting efficient UV-B
resistant mechanisms.
According to the found results, it can be concluded that bacterial communities in the
studied environments are well adapted to high UV-B radiation there is a relationship
bewteen pigmentation and UV-B resistance.