CYTOKINE PRODUCTION IN MESENTERIC LYMPH NODES INDUCED BY YERSINIA ENTEROCOLITICA INFECTION: ROLE OF YOPH

DIAZ M; DAVE MABEL; ELICABE JAVIER; CARGNELUTTI ETHELINA; SILVA JUAN EDUARDO; DI GENARO MARÍA SILVIA

Potrero de los Funes, San Luis

Congreso; Sociedad de Biología de cuyo; 2012

Sociedad de Biología de cuyo

CYTOKINE PRODUCTION IN MESENTERIC LYMPHNODES INDUCED BY Yersinia enterocolitica INFECTION:ROLE OF YOPHDíaz MS, Dave MN, Eliçabe RJ, Cargnelutti E, Silva JE, Di GenaroMS.Laboratory of Immunopathology, National University of San Luis,IMIBIO-SL (CONICET-UNSL). E-mail: sdigena@unsl.edu.arYersinia enterocolitica (Ye) is an enteropathogenic bacterium thatinvades Peyer patches (PP) and may disseminates to mesentericlymph nodes (MLN), spleen (S), lung and liver. YopH is a tyrosinephosphatase secreted by Ye that inhibits the phagocytosis and lymphocyteactivation. The role of YopH during in vivo infection haveyet to be completely elucidated. IFN-γ plays a central role in theimmune response against Ye. The aim was to compare IFN-γ productionin MLN after oral infection with Ye WA-314 (wild-type) orYeΔyopH (YopH deficient). Moreover, we analized the productionof IL-17, a cytokine associated with mucosal immune response.C57BL/6 mice were orally infected with 1?5 108colony formingunits (CFU) of Ye. The CFU in PP, MLN and S were determined 5days after infection. Intracellular cytokine production was analysedin cells from MLN by flow cytometry. We detected lower CFU inPP and MLN after Ye ΔyopH infection (p