Effect of the combined use of cinnamon oil and stevia on the growth

MARÍA F GLIEMMO; CARMEN A. CAMPOS

Lisboa

Conferencia; II International Conference on Antimicrobial Research; 2012

Formatex Research Center

The aim of this study was to evaluate the effect of cinnamon oil and stevia on the growth rate of Z. bailii in acidified aqueous systems resembling low sugar products. A factorial design in three blocks and a central point was performed. The levels of cinnamon oil and stevia were the factors studied. A microtitre plate growth assay was used. The systems were formulated in Sabouraud broth at pH 3.50 and inoculated with Z. bailii reaching a population of 1.106 CFU/ml. The microplate was incubated at 30ºC. Absorbance readings were automatically recorded during the incubation. Growth rates were calculated by using the Logistic model. Cinnamon oil was able to decrease significantly the growth of Z. bailii whereas stevia did not affect it. An antagonistic interaction between both additives was observed. These results suggest that stevia protects the yeast against oil antimicrobial action. A level of cinnamon oil of 0.0056% w/w would be necessary to minimize the growth rate of Z. bailii.