Efecto de Dehidroleucodina en la migración y morfologia de células HeLa

AMAYA M.C; COSTANTINO V.V; BERTOLDI M.V; LOSINNO A.D; LÓPEZ L.A

MENDOZA

Congreso; 26ª Reunión Anual Sociedad Biología de Cuyo; 2010

Sociedad de Biología de Cuyo

For the control of tumor metastasis it is important to identify chemical compounds with antimigratory potency. The present study was designed to examine the effects of a sesquiterpene lactone, Dehydroleucodine (DhL), on the migration of tumor cells in culture. HeLa cells were maintained in DMEM F12 supplemented with 10% fetal bovine serum (FBS). A stock solution of DhL in DMSO at a concentration of 200 mM was prepared. Before use, DhL solution was freshly prepared by diluting with medium to the desired concentration. For wound healing assay culture cells were grown to confluent monolayer in 12-well petri dishes. After 24 hours of incubation in serum-free medium, cells were scratched with a tip and the culture medium was replaced with medium supplemented with 1,5% FBS and hydroxyurea 2 mM (to inhibit proliferation) following the treatment of DhL at different concentration (0, 5, 10 and 20 μM).Wounds were imaged at 0 and 24 h at the same spots by direct microscopic visualization. The cell migration percentages were for Dhl 5 μM 94% ±1,3; 10 μM 77% ±2,9 and 20 μM 56% ±4,7. Wound closure by the control was set to 100%. DhL had been shown to have anti-cancer activity against HeLa cells by inhibiting cell-cycle progression. In this study, we found that DhL could effectively inhibit migration of tumor cells in vitro. DhL could be a new promising candidate as an antimetastatic agent.