INVESTIGADORES
LOSINNO Antonella Denise
congresos y reuniones científicas
Título:
Deshidroleucodina altera la formación de lamelipodios y produce una Migración errática de células HeLa
Autor/es:
AMAYA C.; COSTANTINO V.; LOSINNO A.; BERTOLDI V.; LÓPEZ L.A
Lugar:
San Juan
Reunión:
Congreso; 2da Reunión Conjunta de Sociedades de Biología de la República Argentina; 2011
Institución organizadora:
SBC, SAB, SBCuyo
Resumen:
Metastasis is the main cause of death in patients with cancer, therefore,
it is relevant to find drugs to attack the cell invasion. Previously, we
have demonstrated that Dehydroleucodine (DhL) has an inhibitory
effect in the proliferation of HeLa cells. In this work we studied the
effect of DhL on the migration of HeLa cells. Wound healing assay was
performed in HeLa cells treated with 0 and 20μM DhL. We measured
the speed and direction of migration and analyzed the cytoskeleton by
immunofluorescence. DhL decreased the migration velocity (40%) and
changed the dynamics of the advancing front of the cells. While control
cells generated large and wide lamellipodia and migrated
perpendicularly to the wound, DhL treated cells generated small
lamellipodia and migrated erratically. Also, DhL treatment changed the
organization of the cytoskeleton. In control cells the microtubules were
concentrated in the central region and actin filaments formed an
extensive network in the lamellipodia. In DhL treated cells, the
microtubules were uniformly distributed in the cytoplasm and the actin
filaments were packed in a thin line in the cell periphery. These results
indicate that DhL affects the migration and the cytoskeleton of HeLa
cells. DhL would be a potential agent to stop the invasion of tumor cells
in the body